Overview

  • Product name
  • Description
    Rabbit polyclonal to EIF2S2
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to a region between residues 283 and 333 of Human EIF2S2 (NP_003899.2).

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab86105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 38 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
  • Sequence similarities
    Belongs to the eIF-2-beta/eIF-5 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686L18198 antibody
    • eIF 2 beta antibody
    • eIF-2-beta antibody
    • EIF2 antibody
    • EIF2B antibody
    • EIF2beta antibody
    • EIF2S2 antibody
    • Eukaryotic initiation factor 2 beta antibody
    • eukaryotic initiation factor 2-beta antibody
    • Eukaryotic translation initiation factor 2 beta antibody
    • Eukaryotic translation initiation factor 2 subunit 2 antibody
    • Eukaryotic translation initiation factor 2 subunit 2 beta 38kDa antibody
    • Eukaryotic translation initiation factor 2 subunit 2 beta antibody
    • Eukaryotic translation initiation factor 2 subunit beta antibody
    • eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa antibody
    • IF2B_HUMAN antibody
    • MGC8508 antibody
    • PPP1R67 antibody
    • protein phosphatase 1, regulatory subunit 67 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling EIF2S2 with ab86105 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-EIF2S2 antibody (ab86105) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 38 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 seconds
  • Detection of EIF2S2 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86105 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with exposure time of 10 seconds.

References

ab86105 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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