Overview

  • Product name
  • Description
    Rabbit polyclonal to eIF3A
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 860-910 of Human eIF3A (NP_003741.1; GeneID 8661).

  • Positive control
    • Human Controls: Anaplastic Thyroid Carcinoma, Breast Carcinoma, Colon Carcinoma Mouse Controls: Colon Carcinoma CT26, Renal Cell Carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab105113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.

Target

  • Function
    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Essential for the initiation of translation on type-1 viral ribosomal entry sites (IRESs), like for HCV, PV, EV71 or BEV translation (PubMed:23766293, PubMed:24357634). In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed:18056426).
  • Sequence similarities
    Belongs to the eIF-3 subunit A family.
    Contains 1 PCI domain.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation is enhanced upon serum stimulation.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • centrosomin homolog antibody
    • cytoplasmic protein p167 antibody
    • eIF 3 theta antibody
    • eIF-3-theta antibody
    • EIF3 antibody
    • eIF3 p167 antibody
    • eIF3 p170 antibody
    • eIF3 p180 antibody
    • EIF3 p180 subunit antibody
    • eIF3 p185 antibody
    • eIF3 theta antibody
    • eIF3a antibody
    • EIF3A_HUMAN antibody
    • EIF3S10 antibody
    • eukaryotic translation initiation factor 3 subunit 10 (theta 150 170kD) antibody
    • eukaryotic translation initiation factor 3 subunit 10 (theta 170kD) antibody
    • eukaryotic translation initiation factor 3 subunit 10 170kDa antibody
    • Eukaryotic translation initiation factor 3 subunit 10 antibody
    • eukaryotic translation initiation factor 3 subunit 10 theta 150 170kDa antibody
    • Eukaryotic translation initiation factor 3 subunit A antibody
    • OTTHUMP00000020589 antibody
    • P167 antibody
    • p180 antibody
    • p185 antibody
    • TIF32 antibody
    see all

Images

  • Staining of eIF3A in formalin fixed, paraffin embedded Human Colon Carcinoma by Immunohistochemistry, using ab105113 at a 1/250 dilution.
  • Staining of eIF3A in formalin fixed, paraffin embedded Mouse Renal Cell Carcinoma by Immunohistochemistry, using ab105113 at a 1/250 dilution.

References

ab105113 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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