Anti-eIF3A antibody (ab118357)

Overview

  • Product nameAnti-eIF3A antibody
    See all eIF3A primary antibodies
  • Description
    Mouse monoclonal to eIF3A
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Rabbit, Human
  • Immunogen

    Recombinant fragment corresponding to amino acids 1-76 of Human eIF3A with proprietary 26 kDa tag (NP_003741).

  • Positive control
    • eIF3A transfected HepG2 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab118357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 166 kDa.

Target

  • FunctionComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Essential for the initiation of translation on type-1 viral ribosomal entry sites (IRESs), like for HCV, PV, EV71 or BEV translation (PubMed:23766293, PubMed:24357634). In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed:18056426).
  • Sequence similaritiesBelongs to the eIF-3 subunit A family.
    Contains 1 PCI domain.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation is enhanced upon serum stimulation.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • centrosomin homolog antibody
    • cytoplasmic protein p167 antibody
    • eIF 3 theta antibody
    • eIF-3-theta antibody
    • EIF3 antibody
    • eIF3 p167 antibody
    • eIF3 p170 antibody
    • eIF3 p180 antibody
    • EIF3 p180 subunit antibody
    • eIF3 p185 antibody
    • eIF3 theta antibody
    • eIF3a antibody
    • EIF3A_HUMAN antibody
    • EIF3S10 antibody
    • eukaryotic translation initiation factor 3 subunit 10 (theta 150 170kD) antibody
    • eukaryotic translation initiation factor 3 subunit 10 (theta 170kD) antibody
    • eukaryotic translation initiation factor 3 subunit 10 170kDa antibody
    • Eukaryotic translation initiation factor 3 subunit 10 antibody
    • eukaryotic translation initiation factor 3 subunit 10 theta 150 170kDa antibody
    • Eukaryotic translation initiation factor 3 subunit A antibody
    • OTTHUMP00000020589 antibody
    • P167 antibody
    • p180 antibody
    • p185 antibody
    • TIF32 antibody
    see all

Anti-eIF3A antibody images

  • Anti-eIF3A antibody (ab118357) at 5 µg/ml + eIF3A transfected HepG2 cell lysate at 25 µg

    Predicted band size : 166 kDa
  • Anti-eIF3A antibody (ab118357) at 5 µg/ml + Immunizing peptide at 0.2 µg

    Predicted band size : 166 kDa

References for Anti-eIF3A antibody (ab118357)

ab118357 has not yet been referenced specifically in any publications.

Product Wall

Thank you very much for your valuable feedback.

I added the discount code to our internal records for the Abreview (review28713)and am happy to confirm that code is now valid forpurchasing one freeprimary antibody.

I would like to m...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rabbit Purified protein (0.01-0.05 pmol of purified native eIF3 from rabbit)
Loading amount 0.04 µg
Specification 0.01-0.05 pmol of purified native eIF3 from rabbit
Gel Running Conditions Reduced Denaturing (4-12% BisTris NUPAGE)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Verified customer

Submitted Mar 19 2012

Thank you very much for your inquiry and the testing of ab118357 with rabbit samples.

Unfortunately, we do not have any data on this antibody concerning native protein. The antibody is not yet tested in IP or IHC where we would expect the ant...

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Vielen Dank für Ihre Antwort. Hier ist eine Liste mit den Homologien der Immunogene zu Kaninchen der folgenden Antikörper: eIF3B ABCF1=ABC50 ab40799 100% ab89109 92% ab113218 45% ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"