Overview

  • Product nameAnti-eIF3A antibody
    See all eIF3A primary antibodies
  • Description
    Rabbit polyclonal to eIF3A
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    A region between residue 860 and 910 of human eIF3A using the numbering given in entry NP_003741.1.

  • Positive control
    • HeLa whole cell lysate 293T whole cell lysate NIH3T3 whole cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab86146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 167 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Essential for the initiation of translation on type-1 viral ribosomal entry sites (IRESs), like for HCV, PV, EV71 or BEV translation (PubMed:23766293, PubMed:24357634). In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed:18056426).
  • Sequence similaritiesBelongs to the eIF-3 subunit A family.
    Contains 1 PCI domain.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation is enhanced upon serum stimulation.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • centrosomin homolog antibody
    • cytoplasmic protein p167 antibody
    • eIF 3 theta antibody
    • eIF-3-theta antibody
    • EIF3 antibody
    • eIF3 p167 antibody
    • eIF3 p170 antibody
    • eIF3 p180 antibody
    • EIF3 p180 subunit antibody
    • eIF3 p185 antibody
    • eIF3 theta antibody
    • eIF3a antibody
    • EIF3A_HUMAN antibody
    • EIF3S10 antibody
    • eukaryotic translation initiation factor 3 subunit 10 (theta 150 170kD) antibody
    • eukaryotic translation initiation factor 3 subunit 10 (theta 170kD) antibody
    • eukaryotic translation initiation factor 3 subunit 10 170kDa antibody
    • Eukaryotic translation initiation factor 3 subunit 10 antibody
    • eukaryotic translation initiation factor 3 subunit 10 theta 150 170kDa antibody
    • Eukaryotic translation initiation factor 3 subunit A antibody
    • OTTHUMP00000020589 antibody
    • P167 antibody
    • p180 antibody
    • p185 antibody
    • TIF32 antibody
    see all

Anti-eIF3A antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (left) and mouse teratoma (right) tissues labelling eIF3A with ab86146 at 1/200 (1µg/ml). Detection: DAB.
  • Anti-eIF3A antibody (ab86146) at 1/1000 dilution + Mouse pancreatic cancer whole cell lysate at 20 µg

    Secondary
    IRDye® 680LT-conjugated donkey anti-mouse IgG polyclonal at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 167 kDa
    Observed band size : 166 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Anti-eIF3A antibody (ab86146) at 1/1000 dilution + Human U2OS osteosarcoma whole cell lysate at 20 µg

    Secondary
    IRDye® 800CW conjugated donkey anti-rabbit polyclonal at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 167 kDa
    Observed band size : 166 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

  • All lanes : Anti-eIF3A antibody (ab86146) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 167 kDa


    Exposure time : 3 seconds
  • Detection of Human eIF3A by Immunoprecipitation, using ab86146 at 3µg/mg lysate. Image shows immunoprecipitated eIF3A detected with post IP WB, loading 20% of IP and using HeLa whole cell lysate at 1mg, with an antibody recognising an upstream epitope in lane 1, ab86146 at 1 µg/ml in lane 2 and control IgG in lane 3. Detection: Chemiluminescence with an exposure time of 3 seconds.

References for Anti-eIF3A antibody (ab86146)

This product has been referenced in:
  • Conrad KD  et al. MicroRNA-122 dependent binding of Ago2 protein to hepatitis C virus RNA is associated with enhanced RNA stability and translation stimulation. PLoS One 8:e56272 (2013). IP ; Human . Read more (PubMed: 23405269) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (6%)
Sample Mouse Cell lysate - whole cell (Mouse Pancreatic cancer)
Specification Mouse Pancreatic cancer
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Feb 25 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (6%)
Sample Human Cell lysate - whole cell (U2OS osteosarcoma)
Specification U2OS osteosarcoma
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Feb 21 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"