• Product name
    Anti-eIF3A antibody - N-terminal
    See all eIF3A primary antibodies
  • Description
    Rabbit polyclonal to eIF3A - N-terminal
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 121-347 of Human eIF3A (Uniprot: Q14152)

  • Positive control
    • 293T; A431; Jurkat or Raji cell lysates, Hepatoma tissue, A431 cells
  • General notes
    Keep as a concentrated solution



Our Abpromise guarantee covers the use of ab155412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 166 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.


  • Function
    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Essential for the initiation of translation on type-1 viral ribosomal entry sites (IRESs), like for HCV, PV, EV71 or BEV translation (PubMed:23766293, PubMed:24357634). In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed:18056426).
  • Sequence similarities
    Belongs to the eIF-3 subunit A family.
    Contains 1 PCI domain.
  • Post-translational
    Phosphorylated. Phosphorylation is enhanced upon serum stimulation.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • centrosomin homolog antibody
    • cytoplasmic protein p167 antibody
    • eIF 3 theta antibody
    • eIF-3-theta antibody
    • EIF3 antibody
    • eIF3 p167 antibody
    • eIF3 p170 antibody
    • eIF3 p180 antibody
    • EIF3 p180 subunit antibody
    • eIF3 p185 antibody
    • eIF3 theta antibody
    • eIF3a antibody
    • EIF3A_HUMAN antibody
    • EIF3S10 antibody
    • eukaryotic translation initiation factor 3 subunit 10 (theta 150 170kD) antibody
    • eukaryotic translation initiation factor 3 subunit 10 (theta 170kD) antibody
    • eukaryotic translation initiation factor 3 subunit 10 170kDa antibody
    • Eukaryotic translation initiation factor 3 subunit 10 antibody
    • eukaryotic translation initiation factor 3 subunit 10 theta 150 170kDa antibody
    • Eukaryotic translation initiation factor 3 subunit A antibody
    • OTTHUMP00000020589 antibody
    • P167 antibody
    • p180 antibody
    • p185 antibody
    • TIF32 antibody
    see all


  • Immunofluorescence analysis of methanol fixed A431 cells, labeling eIF3A with ab155412 at 1/200. Lower image shows costaining with Hoechst 33342.
  • Immunohistochemical analysis of paraffin embedded Hepatoma tissue, labeling eIF3A with ab155412 at 1/500.
  • Anti-eIF3A antibody - N-terminal (ab155412) at 1/500 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 166 kDa

    5% SDS PAGE
  • Anti-eIF3A antibody - N-terminal (ab155412) at 1/90000 dilution + 24kDa recombinant eIF3A at 0.1 µg

    Predicted band size: 166 kDa


ab155412 has not yet been referenced specifically in any publications.

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