Overview

  • Product name
  • Description
    Rabbit polyclonal to eIF3B
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 100-150 of Human eIF3B (NP_003742.2)

  • Positive control
    • Human: breast carcinoma; colon carcinoma; non-small cell lung cancer; ovarian carcinoma; prostate carcinoma; stomach adenocarcinoma; testicular seminoma (FFPE sections). Mouse: colon carcinoma CT26; renal cell carcinoma; teratoma (FFPE sections).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, 99% Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab113218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.

Target

  • Function
    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
  • Sequence similarities
    Belongs to the eIF-3 subunit B family.
    Contains 1 RRM (RNA recognition motif) domain.
    Contains 5 WD repeats.
  • Domain
    The RRM domain mediates interaction with EIF3J.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation is enhanced upon serum stimulation.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • eIF-3-eta antibody
    • eIF3 eta antibody
    • eIF3 p110 antibody
    • eIF3 p116 antibody
    • EIF3-ETA antibody
    • EIF3-P110 antibody
    • EIF3-P116 antibody
    • eIF3b antibody
    • EIF3B_HUMAN antibody
    • EIF3S9 antibody
    • EIF3S9, formerly antibody
    • Eukaryotic translation initiation factor 3, subunit B antibody
    • Eukaryotic translation initiation factor 3 subunit 9 antibody
    • eukaryotic translation initiation factor 3 subunit 9 eta antibody
    • Eukaryotic translation initiation factor 3 subunit B antibody
    • eukaryotic translation initiation factor 3, subunit 9 (eta, 116kD) antibody
    • eukaryotic translation initiation factor 3, subunit 9 eta, 116kDa antibody
    • eukaryotic translation initiation factor 3, subunit 9, formerly antibody
    • hPrt1 antibody
    • Protein synthesis defective at 36 degrees celsius 1, S. cerevisiae, homolog of antibody
    • PRT1 antibody
    • Prt1 homolog antibody
    see all

Images

  • ab113218 at a 1/100 dilution staining eIF3B in Human ovarian carcinoma by immunohistochemistry (FFPE section). Secondary antibody used was Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain cross-adsorbed DyLight® 594 Conjugated at a dilution of 1/100.
  • ab113218 at a 1/500 dilution staining eIF3B in Human ovarian carcinoma by immunohistochemistry (FFPE section).
  • ab113218 at a 1/100 dilution staining eIF3B in Mouse teratoma by immunohistochemistry (FFPE section).

References

ab113218 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Vielen Dank für Ihre Antwort. Hier ist eine Liste mit den Homologien der Immunogene zu Kaninchen der folgenden Antikörper: eIF3B ABCF1=ABC50 ab40799 100% ab89109 92% ab113218 45% ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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