Overview

  • Product nameAnti-eIF3B antibody
    See all eIF3B primary antibodies
  • Description
    Rabbit polyclonal to eIF3B
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 100-150 of Human eIF3B (NP_003742.2)

  • Positive control
    • Human: breast carcinoma; colon carcinoma; non-small cell lung cancer; ovarian carcinoma; prostate carcinoma; stomach adenocarcinoma; testicular seminoma (FFPE sections). Mouse: colon carcinoma CT26; renal cell carcinoma; teratoma (FFPE sections).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, 99% Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab113218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.

Target

  • FunctionComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
  • Sequence similaritiesBelongs to the eIF-3 subunit B family.
    Contains 1 RRM (RNA recognition motif) domain.
    Contains 5 WD repeats.
  • DomainThe RRM domain mediates interaction with EIF3J.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation is enhanced upon serum stimulation.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • eIF-3-eta antibody
    • eIF3 eta antibody
    • eIF3 p110 antibody
    • eIF3 p116 antibody
    • EIF3-ETA antibody
    • EIF3-P110 antibody
    • EIF3-P116 antibody
    • eIF3b antibody
    • EIF3B_HUMAN antibody
    • EIF3S9 antibody
    • EIF3S9, formerly antibody
    • Eukaryotic translation initiation factor 3, subunit B antibody
    • Eukaryotic translation initiation factor 3 subunit 9 antibody
    • eukaryotic translation initiation factor 3 subunit 9 eta antibody
    • Eukaryotic translation initiation factor 3 subunit B antibody
    • eukaryotic translation initiation factor 3, subunit 9 (eta, 116kD) antibody
    • eukaryotic translation initiation factor 3, subunit 9 eta, 116kDa antibody
    • eukaryotic translation initiation factor 3, subunit 9, formerly antibody
    • hPrt1 antibody
    • Protein synthesis defective at 36 degrees celsius 1, S. cerevisiae, homolog of antibody
    • PRT1 antibody
    • Prt1 homolog antibody
    see all

Anti-eIF3B antibody images

  • ab113218 at a 1/100 dilution staining eIF3B in Human ovarian carcinoma by immunohistochemistry (FFPE section). Secondary antibody used was Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain cross-adsorbed DyLight® 594 Conjugated at a dilution of 1/100.
  • ab113218 at a 1/500 dilution staining eIF3B in Human ovarian carcinoma by immunohistochemistry (FFPE section).
  • ab113218 at a 1/100 dilution staining eIF3B in Mouse teratoma by immunohistochemistry (FFPE section).

References for Anti-eIF3B antibody (ab113218)

ab113218 has not yet been referenced specifically in any publications.

Product Wall

Vielen Dank für Ihre Antwort. Hier ist eine Liste mit den Homologien der Immunogene zu Kaninchen der folgenden Antikörper: eIF3B ABCF1=ABC50 ab40799 100% ab89109 92% ab113218 45% ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"