Overview

  • Product nameAnti-eIF3B antibody
    See all eIF3B primary antibodies
  • Description
    Rabbit polyclonal to eIF3B
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Drosophila melanogaster
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human eIF3B.

    (Peptide available as ab41603.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates; HEK293, HepG2, SHSY-5Y and U2OS. IF/ICC: HeLa cell line.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab40799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 92 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
  • Sequence similaritiesBelongs to the eIF-3 subunit B family.
    Contains 1 RRM (RNA recognition motif) domain.
    Contains 5 WD repeats.
  • DomainThe RRM domain mediates interaction with EIF3J.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation is enhanced upon serum stimulation.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • eIF-3-eta antibody
    • eIF3 eta antibody
    • eIF3 p110 antibody
    • eIF3 p116 antibody
    • EIF3-ETA antibody
    • EIF3-P110 antibody
    • EIF3-P116 antibody
    • eIF3b antibody
    • EIF3B_HUMAN antibody
    • EIF3S9 antibody
    • EIF3S9, formerly antibody
    • Eukaryotic translation initiation factor 3, subunit B antibody
    • Eukaryotic translation initiation factor 3 subunit 9 antibody
    • eukaryotic translation initiation factor 3 subunit 9 eta antibody
    • Eukaryotic translation initiation factor 3 subunit B antibody
    • eukaryotic translation initiation factor 3, subunit 9 (eta, 116kD) antibody
    • eukaryotic translation initiation factor 3, subunit 9 eta, 116kDa antibody
    • eukaryotic translation initiation factor 3, subunit 9, formerly antibody
    • hPrt1 antibody
    • Protein synthesis defective at 36 degrees celsius 1, S. cerevisiae, homolog of antibody
    • PRT1 antibody
    • Prt1 homolog antibody
    see all

Anti-eIF3B antibody images

  • ICC/IF image of ab40799 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40799, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-eIF3B antibody (ab40799) at 1 µg/ml

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 4 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 92 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    This antibody was raised against an immunogen that is predicted to cross react with both isoforms 1 and 2 of eIF3B. We believe that the band observed is isoform 2 due to its higher molecular weight of 110 kDa. Predicted molecular weights of the isoforms are 92 kDa and 99 kDa respectively.

References for Anti-eIF3B antibody (ab40799)

This product has been referenced in:
  • Fathinajafabadi A  et al. CERKL, a retinal disease gene, encodes an mRNA-binding protein that localizes in compact and untranslated mRNPs associated with microtubules. PLoS One 9:e87898 (2014). WB ; Human . Read more (PubMed: 24498393) »

See 1 Publication for this product

Product Wall

Vielen Dank für Ihre Antwort. Hier ist eine Liste mit den Homologien der Immunogene zu Kaninchen der folgenden Antikörper: eIF3B ABCF1=ABC50 ab40799 100% ab89109 92% ab113218 45% ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"