• Product nameAnti-eIF3B antibody [EPR5805]
    See all eIF3B primary antibodies
  • Description
    Rabbit monoclonal [EPR5805] to eIF3B
  • Tested applicationsSuitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in eIF3B (Human)

  • Positive control
    • WB: 293T, Jurkat, HeLa or Caco 2 cell lysates IHC-P: Human colon tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEPR5805
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab124778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 116 kDa (predicted molecular weight: 92 kDa).
IHC-P 1/50 - 1/100. Antigen retrieval is recommended.
  • Application notesIs unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • FunctionComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
    • Sequence similaritiesBelongs to the eIF-3 subunit B family.
      Contains 1 RRM (RNA recognition motif) domain.
      Contains 5 WD repeats.
    • DomainThe RRM domain mediates interaction with EIF3J.
    • Post-translational
      Phosphorylated. Phosphorylation is enhanced upon serum stimulation.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • eIF-3-eta antibody
      • eIF3 eta antibody
      • eIF3 p110 antibody
      • eIF3 p116 antibody
      • EIF3-ETA antibody
      • EIF3-P110 antibody
      • EIF3-P116 antibody
      • eIF3b antibody
      • EIF3B_HUMAN antibody
      • EIF3S9 antibody
      • EIF3S9, formerly antibody
      • Eukaryotic translation initiation factor 3, subunit B antibody
      • Eukaryotic translation initiation factor 3 subunit 9 antibody
      • eukaryotic translation initiation factor 3 subunit 9 eta antibody
      • Eukaryotic translation initiation factor 3 subunit B antibody
      • eukaryotic translation initiation factor 3, subunit 9 (eta, 116kD) antibody
      • eukaryotic translation initiation factor 3, subunit 9 eta, 116kDa antibody
      • eukaryotic translation initiation factor 3, subunit 9, formerly antibody
      • hPrt1 antibody
      • Protein synthesis defective at 36 degrees celsius 1, S. cerevisiae, homolog of antibody
      • PRT1 antibody
      • Prt1 homolog antibody
      see all

    Anti-eIF3B antibody [EPR5805] images

    • All lanes : Anti-eIF3B antibody [EPR5805] (ab124778) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Caco 2 cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled Goat anti Rabbit at 1/2000 dilution

      Predicted band size : 92 kDa
    • ab124778, at 1/50, staining eIF3B in formalin fixed paraffin embedded Human colon tissue using immunohistochemistry.

    References for Anti-eIF3B antibody [EPR5805] (ab124778)

    ab124778 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab124778.
    Please use the links above to contact us or submit feedback about this product.