Recombinant
RabMAb

Anti-eIF3B antibody [EPR5805] (ab124778)

Overview

  • Product name
    Anti-eIF3B antibody [EPR5805]
    See all eIF3B primary antibodies
  • Description
    Rabbit monoclonal [EPR5805] to eIF3B
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in eIF3B (Human)

  • Positive control
    • WB: 293T, Jurkat, HeLa or Caco 2 cell lysates IHC-P: Human colon tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR5805
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 116 kDa (predicted molecular weight: 92 kDa).
IHC-P 1/50 - 1/100. Antigen retrieval is recommended.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function
      Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
    • Sequence similarities
      Belongs to the eIF-3 subunit B family.
      Contains 1 RRM (RNA recognition motif) domain.
      Contains 5 WD repeats.
    • Domain
      The RRM domain mediates interaction with EIF3J.
    • Post-translational
      modifications
      Phosphorylated. Phosphorylation is enhanced upon serum stimulation.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • eIF-3-eta antibody
      • eIF3 eta antibody
      • eIF3 p110 antibody
      • eIF3 p116 antibody
      • EIF3-ETA antibody
      • EIF3-P110 antibody
      • EIF3-P116 antibody
      • eIF3b antibody
      • EIF3B_HUMAN antibody
      • EIF3S9 antibody
      • EIF3S9, formerly antibody
      • Eukaryotic translation initiation factor 3, subunit B antibody
      • Eukaryotic translation initiation factor 3 subunit 9 antibody
      • eukaryotic translation initiation factor 3 subunit 9 eta antibody
      • Eukaryotic translation initiation factor 3 subunit B antibody
      • eukaryotic translation initiation factor 3, subunit 9 (eta, 116kD) antibody
      • eukaryotic translation initiation factor 3, subunit 9 eta, 116kDa antibody
      • eukaryotic translation initiation factor 3, subunit 9, formerly antibody
      • hPrt1 antibody
      • Protein synthesis defective at 36 degrees celsius 1, S. cerevisiae, homolog of antibody
      • PRT1 antibody
      • Prt1 homolog antibody
      see all

    Images

    • All lanes : Anti-eIF3B antibody [EPR5805] (ab124778) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Caco 2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled Goat anti Rabbit at 1/2000 dilution

      Predicted band size : 92 kDa
    • ab124778, at 1/50, staining eIF3B in formalin fixed paraffin embedded Human colon tissue using immunohistochemistry.

    References

    ab124778 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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