Overview

  • Product nameAnti-EIF3D antibody
    See all EIF3D primary antibodies
  • Description
    Rabbit polyclonal to EIF3D
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from an internal region of Human EIF3D.

  • Positive control
    • NIH3T3 cell extract; Human breast carcinoma tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab109990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 64 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
  • Sequence similaritiesBelongs to the eIF-3 subunit D family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • eIF 3 zeta antibody
    • eIF-3-zeta antibody
    • eIF3 p66 antibody
    • eIF3 zeta antibody
    • eIF3d antibody
    • EIF3D_HUMAN antibody
    • eIF3p66 antibody
    • EIF3S7 antibody
    • Eukaryotic translation initiation factor 3 subunit 7 antibody
    • Eukaryotic translation initiation factor 3 subunit 7 zeta 66/67kDa antibody
    • Eukaryotic translation initiation factor 3 subunit D antibody
    • MGC126526 antibody
    • MGC17258 antibody
    • OTTHUMP00000197900 antibody
    • OTTHUMP00000197901 antibody
    • Translation initiation factor eIF3 p66 subunit antibody
    see all

Anti-EIF3D antibody images

  • ab109990 at 1/50 dilution staining EIF3D in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry. The image on the right is treated with the synthesized peptide.
  • All lanes : Anti-EIF3D antibody (ab109990) at 1/500 dilution

    Lane 1 : NIH3T3 cell extract
    Lane 2 : NIH3T3 cell extract with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 64 kDa

References for Anti-EIF3D antibody (ab109990)

ab109990 has not yet been referenced specifically in any publications.

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