Overview

  • Product name
  • Description
    Rabbit polyclonal to EIF3D
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 241-528 of Human EIF3D (O15371).

  • Positive control
    • HeLaS3, mouse liver, PC12 and Rat2 whole cell lysates. HUH7 xenograft tissue.
  • General notes
    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab155419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 64 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
  • Sequence similarities
    Belongs to the eIF-3 subunit D family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • eIF 3 zeta antibody
    • eIF-3-zeta antibody
    • eIF3 p66 antibody
    • eIF3 zeta antibody
    • eIF3d antibody
    • EIF3D_HUMAN antibody
    • eIF3p66 antibody
    • EIF3S7 antibody
    • Eukaryotic translation initiation factor 3 subunit 7 antibody
    • Eukaryotic translation initiation factor 3 subunit 7 zeta 66/67kDa antibody
    • Eukaryotic translation initiation factor 3 subunit D antibody
    • MGC126526 antibody
    • MGC17258 antibody
    • OTTHUMP00000197900 antibody
    • OTTHUMP00000197901 antibody
    • Translation initiation factor eIF3 p66 subunit antibody
    see all

Images

  • Anti-EIF3D antibody (ab155419) at 1/1000 dilution + HeLa S3 whole cell lysate at 30 µg

    Predicted band size: 64 kDa



    7.5 % SDS-PAGE
  • Anti-EIF3D antibody (ab155419) at 1/5000 dilution + Mouse liver whole cell lysate at 50 µg

    Predicted band size: 64 kDa



    7.5 % SDS-PAGE
  • All lanes : Anti-EIF3D antibody (ab155419) at 1/3000 dilution

    Lane 1 : PC12 whole cell lysate
    Lane 2 : Rat2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 64 kDa



    7.5 % SDS-PAGE
  • Immunohistochemical analysis of paraffin embedded HUH7 xenograft labeling EIF3D with ab155419 antibody at 1/100.

References

This product has been referenced in:
  • He J  et al. High expression of eIF3d is associated with poor prognosis in patients with gastric cancer. Cancer Manag Res 9:539-544 (2017). Read more (PubMed: 29123423) »
  • Lin Y  et al. Eukaryotic translation initiation factor 3 subunit D overexpression is associated with the occurrence and development of ovarian cancer. FEBS Open Bio 6:1201-1210 (2016). WB, IHC . Read more (PubMed: 28203520) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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