Overview

  • Product name
  • Description
    Rabbit polyclonal to EIF3D
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 200 and 250 of human EIF3D (NP_003744.1).

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab85951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
    • Sequence similarities
      Belongs to the eIF-3 subunit D family.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • eIF 3 zeta antibody
      • eIF-3-zeta antibody
      • eIF3 p66 antibody
      • eIF3 zeta antibody
      • eIF3d antibody
      • EIF3D_HUMAN antibody
      • eIF3p66 antibody
      • EIF3S7 antibody
      • Eukaryotic translation initiation factor 3 subunit 7 antibody
      • Eukaryotic translation initiation factor 3 subunit 7 zeta 66/67kDa antibody
      • Eukaryotic translation initiation factor 3 subunit D antibody
      • MGC126526 antibody
      • MGC17258 antibody
      • OTTHUMP00000197900 antibody
      • OTTHUMP00000197901 antibody
      • Translation initiation factor eIF3 p66 subunit antibody
      see all

    Images

    • All lanes : Anti-EIF3D antibody (ab85951) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 64 kDa
      Observed band size : 64 kDa
      Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 3 minutes

    References

    ab85951 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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