Overview

  • Product name
    Anti-eIF3e antibody [EPR6877]
    See all eIF3e primary antibodies
  • Description
    Rabbit monoclonal [EPR6877] to eIF3e
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human eIF3e.

  • Positive control
    • Jurkat, 293T, BxPC-3, A431, Saos 2, HepG2 and NIH 3T3 cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)
    KD = 9.40 x 10 -11 M
    Learn more about KD
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR6877
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab125033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 52 kDa).
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.
    • Tissue specificity
      Ubiquitously expressed. Expressed at highest levels in appendix, lymph, pancreas, skeletal muscle, spleen and thymus.
    • Sequence similarities
      Belongs to the eIF-3 subunit E family.
      Contains 1 PCI domain.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Cytoplasm. Nucleus > PML body.
    • Information by UniProt
    • Database links
    • Alternative names
      • eIF-3 p48 antibody
      • eIF3e antibody
      • EIF3E_HUMAN antibody
      • EIF3S6 antibody
      • eIFe antibody
      • Eukaryotic translation initiation factor 3 subunit 6 antibody
      • Eukaryotic translation initiation factor 3 subunit E antibody
      • eukaryotic translation initiation factor 3, subunit 6 (48kD) antibody
      • INT6 antibody
      • mammary tumor-associated protein INT6 antibody
      • murine mammary tumor integration site 6 (oncogene homolog) antibody
      • Viral integration site protein INT-6 homolog antibody
      see all

    Images

    • All lanes : Anti-eIF3e antibody [EPR6877] (ab125033) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : BxPC3 cell lysate
      Lane 4 : A431 cell lysate
      Lane 5 : Saos 2 cell lysate
      Lane 6 : Jurkat cell lysate
      Lane 7 : NIH 3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size : 52 kDa
      Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Hoedt E  et al. SILAC-based proteomic profiling of the human MDA-MB-231 metastatic breast cancer cell line in response to the two antitumoral lactoferrin isoforms: the secreted lactoferrin and the intracellular delta-lactoferrin. PLoS One 9:e104563 (2014). WB ; Human . Read more (PubMed: 25116916) »

    See 1 Publication for this product

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    There are currently no Customer reviews or Questions for ab125033.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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