Anti-EIF3F antibody (ab155475)
Key features and details
- Rabbit polyclonal to EIF3F
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EIF3F antibody
See all EIF3F primary antibodies -
Description
Rabbit polyclonal to EIF3F -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Recombinant fragment corresponding to a region within amino acids 55-328 of Human EIF3F (Uniprot ID: O00303).
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Positive control
- HeLa whole cell lysate (ab150035). HeLa cells. Human BT483 xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155475 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 38 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 38 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0). |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Deubiquitinates activated NOTCH1, promoting its nuclear import, thereby acting as a positive regulator of Notch signaling. -
Sequence similarities
Belongs to the eIF-3 subunit F family.
Contains 1 MPN (JAB/Mov34) domain. -
Domain
The MPN domain mediates deubiquitinating activity. -
Post-translational
modificationsPhosphorylation is enhanced upon serum stimulation. Phosphorylated during apoptosis by caspase-processed CDK11. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 8665 Human
- Entrez Gene: 66085 Mouse
- Omim: 603914 Human
- SwissProt: O00303 Human
- SwissProt: Q9DCH4 Mouse
- Unigene: 516023 Human
- Unigene: 182962 Mouse
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Alternative names
- Deubiquitinating enzyme eIF3f antibody
- eIF 3 epsilon antibody
- eIF-3-epsilon antibody
see all
Images
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Anti-EIF3F antibody (ab155475) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 38 kDa
10% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded Human BT483 xenograft tissue, labeling EIF3F using ab155475 at a 1/100 dilution.
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Immunofluorescence analysis of methanol-fixed HeLa cells, labeling EIF3F using ab155475 at a 1/200 dilution. The image in the lower panel was costained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab155475 has been referenced in 1 publication.
- Esteves P et al. Nuclear control of lung cancer cells migration, invasion and bioenergetics by eukaryotic translation initiation factor 3F. Oncogene 39:617-636 (2020). PubMed: 31527668