Overview

  • Product name
  • Description
    Rabbit polyclonal to EIF3F
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide within Human EIF3F aa 307-357 (C terminal). The exact sequence is proprietary. NP_003745.1
    Sequence:

    FLMS LVNQVPKIVP DDFETMLNSN INDLLMVTYL ANLTQSQIAL NEKLVNL


    Database link: O00303

  • Positive control
    • HeLa and NIH 3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab176853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 38 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
    Deubiquitinates activated NOTCH1, promoting its nuclear import, thereby acting as a positive regulator of Notch signaling.
  • Sequence similarities
    Belongs to the eIF-3 subunit F family.
    Contains 1 MPN (JAB/Mov34) domain.
  • Domain
    The MPN domain mediates deubiquitinating activity.
  • Post-translational
    modifications
    Phosphorylation is enhanced upon serum stimulation. Phosphorylated during apoptosis by caspase-processed CDK11.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deubiquitinating enzyme eIF3f antibody
    • eIF 3 epsilon antibody
    • eIF-3-epsilon antibody
    • eIF3 p47 antibody
    • eIF3 p47 subunit antibody
    • eIF3-epsilon antibody
    • eIF3-p47 antibody
    • eIF3f antibody
    • EIF3F_HUMAN antibody
    • EIF3S5 antibody
    • EIF3S5, formerly antibody
    • Eukaryotic translation initiation factor 3 subunit 5 antibody
    • Eukaryotic translation initiation factor 3 subunit 5, formerly antibody
    • Eukaryotic translation initiation factor 3 subunit F antibody
    • eukaryotic translation initiation factor 3, subunit 5 (epsilon, 47kD) antibody
    • eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa antibody
    • eukaryotic translation initiation factor 3, subunit F antibody
    • translation initiation factor 3 47 kda subunit antibody
    see all

Images

  • All lanes : Anti-EIF3F antibody (ab176853) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : NIH 3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 38 kDa


    Exposure time : 3 minutes
  • Detection of EIF3F in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176853 at 6 µg/mg lysate for IP (Lane 1). For WB detection, ab176853 was used at 0.4 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.

References

This product has been referenced in:
  • Son YH  et al. Dexamethasone downregulates caveolin-1 causing muscle atrophy via inhibited insulin signaling. J Endocrinol 225:27-37 (2015). WB ; Mouse . Read more (PubMed: 25688118) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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