Overview

  • Product nameAnti-EIF3F antibody
    See all EIF3F primary antibodies
  • Description
    Rabbit polyclonal to EIF3F
  • Tested applicationsSuitable for: WB, IP, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Chimpanzee
    Predicted to work with: Cynomolgus Monkey
  • Immunogen

    Synthetic peptide:

    ARVIGTLLGTVD

    , corresponding to amino acids 114-125 of Human EIF3F

  • Positive control
    • Human brain cerebellum tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab64177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/2000. Predicted molecular weight: 38 kDa.
IP Use at an assay dependent concentration.
ELISA 1/10000 - 1/40000.
IHC-P Use a concentration of 2.5 µg/ml.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
    Deubiquitinates activated NOTCH1, promoting its nuclear import, thereby acting as a positive regulator of Notch signaling.
  • Sequence similaritiesBelongs to the eIF-3 subunit F family.
    Contains 1 MPN (JAB/Mov34) domain.
  • DomainThe MPN domain mediates deubiquitinating activity.
  • Post-translational
    modifications
    Phosphorylation is enhanced upon serum stimulation. Phosphorylated during apoptosis by caspase-processed CDK11.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deubiquitinating enzyme eIF3f antibody
    • eIF 3 epsilon antibody
    • eIF-3-epsilon antibody
    • eIF3 p47 antibody
    • eIF3 p47 subunit antibody
    • eIF3-epsilon antibody
    • eIF3-p47 antibody
    • eIF3f antibody
    • EIF3F_HUMAN antibody
    • EIF3S5 antibody
    • EIF3S5, formerly antibody
    • Eukaryotic translation initiation factor 3 subunit 5 antibody
    • Eukaryotic translation initiation factor 3 subunit 5, formerly antibody
    • Eukaryotic translation initiation factor 3 subunit F antibody
    • eukaryotic translation initiation factor 3, subunit 5 (epsilon, 47kD) antibody
    • eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa antibody
    • eukaryotic translation initiation factor 3, subunit F antibody
    • translation initiation factor 3 47 kda subunit antibody
    see all

Anti-EIF3F antibody images

  • ab64177 at 2.5µg/ml staining EIF3F in human brain cerebellum by Immunohistochemistry, Formalin-fixed, paraffin-embedded tissue.
  • ICC/IF image of ab64177 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64177, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-EIF3F antibody (ab64177)

This product has been referenced in:
  • Lang CH  et al. BCATm deficiency ameliorates endotoxin-induced decrease in muscle protein synthesis and improves survival in septic mice. Am J Physiol Regul Integr Comp Physiol 299:R935-44 (2010). Read more (PubMed: 20554928) »

See 1 Publication for this product

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