Overview

  • Product name
  • Description
    Rabbit polyclonal to eIF3K
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A recombinant fragment corresponding to a region within amino acids 41 - 218 of Human eIF3K (Uniprot ID Q9UBQ5).

  • Positive control
    • 293T cell lysate; Cal27 xenograft ; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab153985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 25 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. 10mM Citrate buffer
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
  • Tissue specificity
    Ubiquitous, with the highest levels of expression in brain, testis and kidney.
  • Sequence similarities
    Belongs to the eIF-3 subunit K family.
  • Cellular localization
    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARG134 antibody
    • eIF 3 p25 antibody
    • eIF 3 p28 antibody
    • eIF-3 p25 antibody
    • eIF-3 p28 antibody
    • EIF3-p28 antibody
    • eif3k antibody
    • EIF3K_HUMAN antibody
    • EIF3S12 antibody
    • Eukaryotic translation initiation factor 3 subunit 12 antibody
    • Eukaryotic translation initiation factor 3 subunit K antibody
    • HSPC029 antibody
    • M9 antibody
    • MSTP001 antibody
    • Muscle specific gene M9 protein antibody
    • Muscle-specific gene M9 protein antibody
    • PLAC 24 antibody
    • PLAC-24 antibody
    • PLAC24 antibody
    • PRO1474 antibody
    • PTD001 antibody
    see all

Images

  • Anti-eIF3K antibody (ab153985) at 1/1000 dilution + 293T cell lysate at 30 µg

    Predicted band size : 25 kDa
  • Immunohistochemical analysis of paraffin embedded Cal27 xenograft labeling eIF3K using ab153985 at 1/100 dilution.
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling eIF3K using ab153985 at 1/500 dilution. Right panel costained with Hoechst 33342.

References

ab153985 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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