Overview

  • Product nameAnti-EIF3S1 antibody
    See all EIF3S1 primary antibodies
  • Description
    Mouse polyclonal to EIF3S1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length EIF3S1 protein (Human)

  • Positive control
    • EIF3S1 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab68243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 29 kDa).

Target

  • FunctionComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit binds directly within the mRNA entry channel of the 40S ribosome to the aminoacyl (A) site. It may regulate the interaction between the 43S PIC and mRNA.
  • Sequence similaritiesBelongs to the eIF-3 subunit J family.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation is enhanced upon serum stimulation.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • eIF 3 alpha antibody
    • eIF-3-alpha antibody
    • eIF3 alpha antibody
    • eIF3 p35 antibody
    • eIF3j antibody
    • EIF3J_HUMAN antibody
    • EIF3S 1 antibody
    • EIF3S1 antibody
    • Eukaryotic translation initiation factor 3 subunit 1 antibody
    • Eukaryotic translation initiation factor 3 subunit J antibody
    • Eukaryotic translation initiation factor 3, subunit 1 (alpha, 35kD) antibody
    • Eukaryotic translation initiation factor 3, subunit 1 alpha, 35kD antibody
    see all

Anti-EIF3S1 antibody images

  • All lanes : Anti-EIF3S1 antibody (ab68243) at 1/500 dilution

    Lane 1 : EIF3S1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 29 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

References for Anti-EIF3S1 antibody (ab68243)

ab68243 has not yet been referenced specifically in any publications.

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