Anti-EIF3S1 antibody - Carboxyterminal end (ab71416)

Overview

  • Product nameAnti-EIF3S1 antibody - Carboxyterminal end
    See all EIF3S1 primary antibodies
  • Description
    Rabbit polyclonal to EIF3S1 - Carboxyterminal end
  • Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human) from the C-terminal region of human EIF3S1, conjugated to KLH.

  • Positive control
    • K562 cell line lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab71416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50.
WB 1/50 - 1/100. Detects a band of approximately 36 kDa (predicted molecular weight: 29 kDa).
ELISA 1/1000.

Target

  • FunctionComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit binds directly within the mRNA entry channel of the 40S ribosome to the aminoacyl (A) site. It may regulate the interaction between the 43S PIC and mRNA.
  • Sequence similaritiesBelongs to the eIF-3 subunit J family.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation is enhanced upon serum stimulation.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • eIF 3 alpha antibody
    • eIF-3-alpha antibody
    • eIF3 alpha antibody
    • eIF3 p35 antibody
    • eIF3j antibody
    • EIF3J_HUMAN antibody
    • EIF3S 1 antibody
    • EIF3S1 antibody
    • Eukaryotic translation initiation factor 3 subunit 1 antibody
    • Eukaryotic translation initiation factor 3 subunit J antibody
    • Eukaryotic translation initiation factor 3, subunit 1 (alpha, 35kD) antibody
    • Eukaryotic translation initiation factor 3, subunit 1 alpha, 35kD antibody
    see all

Anti-EIF3S1 antibody - Carboxyterminal end images

  • Anti-EIF3S1 antibody - Carboxyterminal end (ab71416) at 1/60 dilution + K562 cell line lysates at 35 µg

    Predicted band size : 29 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • ab71416 at 1/50 dilution staining EIF3S1 - Carboxyterminal end in human lung carcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

References for Anti-EIF3S1 antibody - Carboxyterminal end (ab71416)

ab71416 has not yet been referenced specifically in any publications.

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