Recombinant
RabMAb

Anti-EIF3S1 antibody [EPR16161] (ab196018)

Overview

  • Product name
    Anti-EIF3S1 antibody [EPR16161]
    See all EIF3S1 primary antibodies
  • Description
    Rabbit monoclonal [EPR16161] to EIF3S1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human EIF3S1 aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: O75822

  • Positive control
    • HeLa and K562 cell lysates; Human chronic tonsillitis, Human transitional cell carcinoma of bladder and Rat cerebral cortex tissues; HeLa cells; K562 cells; HeLa whole cell extract.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab196018 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000. Detects a band of approximately 35 kDa (predicted molecular weight: 29 kDa).
ICC/IF 1/180.
Flow Cyt 1/170.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.

Target

  • Function
    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit binds directly within the mRNA entry channel of the 40S ribosome to the aminoacyl (A) site. It may regulate the interaction between the 43S PIC and mRNA.
  • Sequence similarities
    Belongs to the eIF-3 subunit J family.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation is enhanced upon serum stimulation.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • eIF 3 alpha antibody
    • eIF-3-alpha antibody
    • eIF3 alpha antibody
    • eIF3 p35 antibody
    • eIF3j antibody
    • EIF3J_HUMAN antibody
    • EIF3S 1 antibody
    • EIF3S1 antibody
    • Eukaryotic translation initiation factor 3 subunit 1 antibody
    • Eukaryotic translation initiation factor 3 subunit J antibody
    • Eukaryotic translation initiation factor 3, subunit 1 (alpha, 35kD) antibody
    • Eukaryotic translation initiation factor 3, subunit 1 alpha, 35kD antibody
    see all

Images

  • All lanes : Anti-EIF3S1 antibody [EPR16161] (ab196018) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate
    Lane 2 : K562 (Human chronic myelogenous leukemia cells from bone marrow) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 29 kDa
    Observed band size: 35 kDa (why is the actual band size different from the predicted?)



    Blocking/Dilution buffer: 5% NFDM/TBST.

    A band is detected at 35 kDa. While this differs to its predicted molecular weight of 29 kDa, this migration has been observed in the literature and may be due to glycosylation (PMID: 8995409)

  • Immunohistochemical analysis of paraffin-embedded Human chronic tonsillitis tissue labeling EIF3S1 with ab196018 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Human chronic tonsillitis tissue is observed. Counter stained with Hematoxylin.

    Secondary control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Capture antibody ab196018 at 1/50.



    All lanes : Anti-EIF3S1 antibody [EPR16161] (ab196018) at 1/1000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : Rabbit monoclonal IgG instead of ab196018 in HeLa lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling EIF3S1 with ab196018 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Human transitional cell carcinoma of bladder tissue is observed. Counter stained with Hematoxylin.

    Secondary control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling EIF3S1 with ab196018 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Rat cerebral cortex is observed. Counter stained with Hematoxylin.

    Secondary control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling EIF3S1 with ab196018 at 1/170 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

  • EIF3S1 was immunoprecipitated from HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab196018 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab196018 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1000 dilution.

    Lane 1: HeLa whole cell extract 10 µg (Input). Lane 2: ab196018 IP in HeLa whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab196018 in HeLa whole cell extract.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

References

ab196018 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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