Overview

  • Product name
  • Description
    Rabbit polyclonal to EIF3S2
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 66-325 of Human EIF3S2 (Uniprot ID: Q13347).

  • Positive control
    • A431, H1299, HeLa and HepG2 whole cell lysates. HeLa cells.
  • General notes
    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab155477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 37 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
  • Sequence similarities
    Belongs to the eIF-3 subunit I family.
    Contains 5 WD repeats.
  • Post-translational
    modifications
    Phosphorylated by TGF-beta type II receptor.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • eIF-3-beta antibody
    • eIF3 beta antibody
    • eIF3 p36 antibody
    • eIF3-beta antibody
    • eIF3-p36 antibody
    • eIF3i antibody
    • EIF3I_HUMAN antibody
    • EIF3S2 antibody
    • EIF3S2, formerly antibody
    • Eukaryotic translation initiation factor 3 subunit 2 antibody
    • Eukaryotic translation initiation factor 3 subunit 2 beta antibody
    • Eukaryotic translation initiation factor 3 subunit I antibody
    • eukaryotic translation initiation factor 3, subunit 2 (beta, 36kD antibody
    • eukaryotic translation initiation factor 3, subunit 2 beta, 36kDa antibody
    • eukaryotic translation initiation factor 3, subunit 2, formerly antibody
    • eukaryotic translation initiation factor 3, subunit I antibody
    • Predicted protein of HQ2242 antibody
    • PRO2242 antibody
    • TGF beta receptor interacting protein 1 antibody
    • TGF-beta receptor-interacting protein 1 antibody
    • TGFbeta receptor interacting protein 1 antibody
    • TGFbeta receptor-interacting protein 1 antibody
    • TRIP 1 antibody
    • TRIP-1 antibody
    • TRIP1 antibody
    see all

Images

  • All lanes : Anti-EIF3S2 antibody (ab155477) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 37 kDa
  • Immunofluorescence analysis of methanol-fixed HeLa cells, labeling EIF3S2 using ab155477 at a 1/200 dilution. The image in the lower panel was costained with Hoechst 33342.

References

ab155477 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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