Overview

  • Product nameAnti-EIF3S3 antibody
    See all EIF3S3 primary antibodies
  • Description
    Mouse monoclonal to EIF3S3
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment with tag: YDPIKTAQGS LSLKAYRLTP KLMEVCKEKD FSPEALKKAN ITFEYMFEEV PIVIKNSHLI NVLMWELEKK SAVADKHELL SLASSNHLGK NLQLLMDRV, corresponding to amino acids 152-251 of Human EIF3S3

  • Positive control
    • Jurkat whole cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab60942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Detects a band of approximately 37 kDa (predicted molecular weight: 40 kDa).
ELISA Use at an assay dependent dilution.

Target

  • FunctionComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
  • Sequence similaritiesBelongs to the eIF-3 subunit H family.
    Contains 1 MPN (JAB/Mov34) domain.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • eIF-3-gamma antibody
    • eIF3 gamma antibody
    • eIF3 p40 antibody
    • eIF3 p40 subunit antibody
    • eIF3h antibody
    • EIF3H_HUMAN antibody
    • EIF3S3 antibody
    • Eukaryotic translation initiation factor 3 subunit 3 antibody
    • Eukaryotic translation initiation factor 3 subunit H antibody
    • MGC102958 antibody
    see all

Anti-EIF3S3 antibody images

  • Anti-EIF3S3 antibody (ab60942) + Jurkat whole cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 40 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

References for Anti-EIF3S3 antibody (ab60942)

ab60942 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"