• Product nameAnti-EIF3S4 antibody
    See all EIF3S4 primary antibodies
  • Description
    Rabbit polyclonal to EIF3S4
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    A region within synthetic peptide: ATGDTSPEPE LLPGAPLPPP KEVINGNIKT VTEYKIDEDG KKFKIVRTFR, corresponding to N terminal amino acids 37-86 of Human EIF3S4

  • Positive control
    • WB: HepG2 cell lysate. IHC-P: Human kidney tissue.



Our Abpromise guarantee covers the use of ab50964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.


  • FunctionComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit can bind 18S rRNA.
  • Sequence similaritiesBelongs to the eIF-3 subunit G family.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    Phosphorylated. Phosphorylation is enhanced upon serum stimulation.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasm > perinuclear region. Colocalizes with AIFM1 in the nucleus and perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • 44kDa antibody
    • Anti EIF3S4 antibody
    • eIF 3 delta antibody
    • eIF 3 RNA binding subunit antibody
    • eIF-3 RNA-binding subunit antibody
    • eIF-3-delta antibody
    • eIF3 delta antibody
    • eIF3 p42 antibody
    • eIF3 p44 antibody
    • eIF3g antibody
    • EIF3G_HUMAN antibody
    • EIF3S4 antibody
    • EIF3S4 antibody antibody
    • EIF3S4, formerly antibody
    • Eukaryotic translation initiation factor 3 antibody
    • Eukaryotic translation initiation factor 3 RNA binding subunit antibody
    • Eukaryotic translation initiation factor 3 RNA-binding subunit antibody
    • Eukaryotic translation initiation factor 3 subunit 4 antibody
    • Eukaryotic translation initiation factor 3 subunit 4 delta 44 kD antibody
    • Eukaryotic translation initiation factor 3 subunit G antibody
    • Eukaryotic translation initiation factor 3 subunit p42 antibody
    • Eukaryotic translation initiation factor 3, subunit 4, formerly antibody
    • Subunit 4 (delta 44kD) antibody
    • Subunit 4 delta antibody
    see all

Anti-EIF3S4 antibody images

  • Lane 1 : Marker
    Lane 2 : Anti-EIF3S4 antibody (ab50964) at 1.25 µg/ml

    Lane 1 : As above
    Lane 2 : HepG2 cell lysate at 10 µg

    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 36 kDa
    Observed band size : 41 kDa (why is the actual band size different from the predicted?)
  • ab50964, at a concentration of 4 µg/ml, staining EIF3S4 in paraffin embedded human kidney sections by Immunohistochemistry. Positively labeled cells (epithelial cells of renal tubule) are indicated with arrows. Magnification 400x.

References for Anti-EIF3S4 antibody (ab50964)

ab50964 has not yet been referenced specifically in any publications.

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