The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1.25 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 4 - 8 µg/ml.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/312500.
FunctionComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit can bind 18S rRNA.
Sequence similaritiesBelongs to the eIF-3 subunit G family. Contains 1 RRM (RNA recognition motif) domain.
Post-translational modificationsPhosphorylated. Phosphorylation is enhanced upon serum stimulation.
Cellular localizationCytoplasm. Nucleus. Cytoplasm > perinuclear region. Colocalizes with AIFM1 in the nucleus and perinuclear region.
ab50964, at a concentration of 4 µg/ml, staining EIF3S4 in paraffin embedded human kidney sections by Immunohistochemistry. Positively labeled cells (epithelial cells of renal tubule) are indicated with arrows. Magnification 400x.
References for Anti-EIF3S4 antibody (ab50964)
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