Overview

  • Product nameAnti-EIF3S4 antibody
    See all EIF3S4 primary antibodies
  • Description
    Rabbit polyclonal to EIF3S4
  • Tested applicationsSuitable for: ELISA, ICC/IF, IP, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Goat, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide: PSLRDGASRR GESMQPNRRA DDNATIRVTN LSEDTRETDL QELFRPFGSI , corresponding to a region within internal sequence amino acids 216 - 265 of Human EIF3S4 (NP_003746)

  • Positive control
    • 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

ICC/IF 1/500.
IP 1/500.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit can bind 18S rRNA.
  • Sequence similaritiesBelongs to the eIF-3 subunit G family.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation is enhanced upon serum stimulation.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasm > perinuclear region. Colocalizes with AIFM1 in the nucleus and perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • 44kDa antibody
    • Anti EIF3S4 antibody
    • eIF 3 delta antibody
    • eIF 3 RNA binding subunit antibody
    • eIF-3 RNA-binding subunit antibody
    • eIF-3-delta antibody
    • eIF3 delta antibody
    • eIF3 p42 antibody
    • eIF3 p44 antibody
    • eIF3g antibody
    • EIF3G_HUMAN antibody
    • EIF3S4 antibody
    • EIF3S4 antibody antibody
    • EIF3S4, formerly antibody
    • Eukaryotic translation initiation factor 3 antibody
    • Eukaryotic translation initiation factor 3 RNA binding subunit antibody
    • Eukaryotic translation initiation factor 3 RNA-binding subunit antibody
    • Eukaryotic translation initiation factor 3 subunit 4 antibody
    • Eukaryotic translation initiation factor 3 subunit 4 delta 44 kD antibody
    • Eukaryotic translation initiation factor 3 subunit G antibody
    • Eukaryotic translation initiation factor 3 subunit p42 antibody
    • Eukaryotic translation initiation factor 3, subunit 4, formerly antibody
    • Subunit 4 (delta 44kD) antibody
    • Subunit 4 delta antibody
    see all

Anti-EIF3S4 antibody images

  • Anti-EIF3S4 antibody (ab83415) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 36 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence analysis of Human brain stem cells staining EIF3S4 with ab83415 at 2 µg/ml.

References for Anti-EIF3S4 antibody (ab83415)

ab83415 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"