Recombinant Anti-eIF3g antibody [EPR16147] (ab192601)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16147] to eIF3g
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-eIF3g antibody [EPR16147]
See all eIF3g primary antibodies -
Description
Rabbit monoclonal [EPR16147] to eIF3g -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293T, MOLT4, K562, mouse Raw 264.7, mouse NIH 3T3 and rat PC12 cell lysates. HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16147 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192601 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/2000. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa).
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ICC/IF |
1/100.
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IP |
1/40.
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Notes |
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WB
1/1000 - 1/2000. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa). |
ICC/IF
1/100. |
IP
1/40. |
Target
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Function
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit can bind 18S rRNA. -
Sequence similarities
Belongs to the eIF-3 subunit G family.
Contains 1 RRM (RNA recognition motif) domain. -
Post-translational
modificationsPhosphorylated. Phosphorylation is enhanced upon serum stimulation. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasm > perinuclear region. Colocalizes with AIFM1 in the nucleus and perinuclear region. - Information by UniProt
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Database links
- Entrez Gene: 8666 Human
- Entrez Gene: 53356 Mouse
- Entrez Gene: 298700 Rat
- Omim: 603913 Human
- SwissProt: O75821 Human
- SwissProt: Q9Z1D1 Mouse
- SwissProt: Q5RK09 Rat
- Unigene: 529059 Human
see all -
Alternative names
- 44kDa antibody
- Anti EIF3S4 antibody
- eIF 3 delta antibody
see all
Images
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All lanes : Anti-eIF3g antibody [EPR16147] (ab192601) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : MOLT-4 cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 36 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted? -
All lanes : Anti-eIF3g antibody [EPR16147] (ab192601) at 1/1000 dilution
Lane 1 : mouse Raw 264.7 cell lysate
Lane 2 : rat PC12 cell lysate
Lane 3 : mouse NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 36 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted? -
Immunofluorescence analysis of, paraformaldehyde-fixed, HeLa cells labeling eIF3g with ab192601 at a 1/100 dilution ( 7.0 μg/ml). As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) ab150077 was used at a 1/400 dilution. In blue DAPI staining.
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Western blot analysis on immunoprecipitation from 1) K562 cell lysate and 2) PBS, labeling eIF3g using ab192601 at 1/40 dilution and HRP-conjugated anti-rabbit IgG specific to the non-reduced form of rabbit IgG at a 1/1500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab192601 has not yet been referenced specifically in any publications.