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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human eIF4A1.
(Peptide available as ab31796.)
Our Abpromise guarantee covers the use of ab31217 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).|
|IP||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Image courtesy of Human Protein Atlas. ab31217 staining eIF4A1 in human stomach, showing strong cytoplasmic staining in the glandular cells. Paraffin embedded human stomach tissue was incubated with ab31217 (1/100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab31217 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues. Further results for this antibody can be found at www.proteinatlas.org