Anti-eIF4A1 antibody - N-terminal (ab71252)

Overview

  • Product nameAnti-eIF4A1 antibody - N-terminal
    See all eIF4A1 primary antibodies
  • Description
    Rabbit polyclonal to eIF4A1 - N-terminal
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from the N-terminal region of human eIF4A1 conjugated to KLH

  • Positive control
    • K562 cell line lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab71252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/50 - 1/100. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
IHC-P 1/10 - 1/50.

Target

  • FunctionATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
  • Sequence similaritiesBelongs to the DEAD box helicase family. eIF4A subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP-dependent RNA helicase eIF4A-1 antibody
    • DDX2 antibody
    • DDX2A antibody
    • eIF 4A I antibody
    • eIF-4A-I antibody
    • EIF4A antibody
    • eIF4A I antibody
    • eIF4A-I antibody
    • EIF4A1 antibody
    • Eukaryotic initiation factor 4A-I antibody
    • Eukaryotic initiation factor 4AI antibody
    • eukaryotic translation initiation factor 4A, isoform 1 antibody
    • Eukaryotic translation initiation factor 4A1 antibody
    • IF4A1_HUMAN antibody
    see all

Anti-eIF4A1 antibody - N-terminal images

  • Anti-eIF4A1 antibody - N-terminal (ab71252) at 1/60 dilution + K562 cell line lysate at 35 µg

    Predicted band size : 47 kDa
    Observed band size : 47 kDa
  • ab71252 at 1/50 dilution staining elF4A1 in human lung carcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

References for Anti-eIF4A1 antibody - N-terminal (ab71252)

ab71252 has not yet been referenced specifically in any publications.

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