Overview

  • Product nameAnti-eIF4A2 antibody
    See all eIF4A2 primary antibodies
  • Description
    Rabbit polyclonal to eIF4A2
  • Tested applicationsSuitable for: IHC-P, IP, WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human eIF4A2.

    (Peptide available as ab32473.)

  • Positive control
    • Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Brain (Mouse) Tissue Lysate Testis (Mouse) Tissue Lysate Skeletal Muscle (Mouse) Tissue Lysate (ab29711) Spinal Cord (Mouse) Tissue Lysate Ovary (Mouse) Tissue Lysate PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate Brain (Rat) Tissue Lysate Heart (Rat) Tissue Lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab31218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
ICC 1/200.

Target

  • FunctionATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
  • Sequence similaritiesBelongs to the DEAD box helicase family. eIF4A subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP dependent RNA helicase eIF4A 2 antibody
    • ATP-dependent RNA helicase eIF4A-2 antibody
    • BM-010 antibody
    • DDX2B antibody
    • eIF 4A II antibody
    • eIF-4A-II antibody
    • EIF4A antibody
    • eIF4A II antibody
    • eIF4A-II antibody
    • EIF4A2 antibody
    • EIF4F antibody
    • Eukaryotic initiation factor 4A II antibody
    • Eukaryotic initiation factor 4A-II antibody
    • eukaryotic translation initiation factor 4A isoform 2 antibody
    • Eukaryotic translation initiation factor 4A2 antibody
    • IF4A2_HUMAN antibody
    • N-terminally processed antibody
    see all

Anti-eIF4A2 antibody images

  • Anti-eIF4A2 antibody (ab31218) at 1 µg/ml + Jurkat whole cell lysate (ab7899) at 20 µg

    Secondary
    IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 47 kDa
    Additional bands at : 52 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-eIF4A2 antibody (ab31218) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Testis (Mouse) Tissue Lysate
    Lane 3 : Mouse skeletal muscle tissue lysate - total protein (ab29711)
    Lane 4 : Spinal Cord (Mouse) Tissue Lysate
    Lane 5 : Ovary (Mouse) Tissue Lysate
    Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 7 : Brain (Rat) Tissue Lysate
    Lane 8 : Heart (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 47 kDa
    Additional bands at : 51 kDa. We are unsure as to the identity of these extra bands.
  • Image courtesy of Human Protein Atlas. ab31218 staining eIF4A2 in human duodenum, showing strong cytomplasmic staining in the villi and Brunner's gland cells. Paraffin embedded human duodenum tissue was incubated with ab31218 (1/400 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab31218 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues. Further results for this antibody can be found at www.proteinatlas.org

  • Mineralocorticoid Receptor was immunoprecipitated using 0.5mg Mouse skeletal muscle whole tissue extract, 5µg of Rabbit polyclonal to Mineralocorticoid Receptor and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse skeletal muscle whole tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab31218.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 47kDa: Mineralocorticoid Receptor.

References for Anti-eIF4A2 antibody (ab31218)

This product has been referenced in:
  • Galicia-Vázquez G  et al. Regulation of eukaryotic initiation factor 4AII by MyoD during murine myogenic cell differentiation. PLoS One 9:e87237 (2014). WB ; Mouse . Read more (PubMed: 24466343) »
  • Menalled LB  et al. Genetic Deletion of Transglutaminase 2 Does Not Rescue the Phenotypic Deficits Observed in R6/2 and zQ175 Mouse Models of Huntington's Disease. PLoS One 9:e99520 (2014). WB ; Mouse . Read more (PubMed: 24955833) »

See all 9 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (hek293, hela)
Loading amount 50 µg
Specification hek293, hela
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Feb 25 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Loading amount 40 µg
Specification Mouse embryonic fibroblasts
Gel Running Conditions Reduced Denaturing (7.5)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Feb 24 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (MDA-MB231 and Hela Cell lines)
Loading amount 50000 cells
Specification MDA-MB231 and Hela Cell lines
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jul 05 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cultured Cells (MDA-MB231 Cells)
Specification MDA-MB231 Cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% Saponin
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jul 04 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"