For the best experience on the Abcam website please upgrade to a modern browser such as
Storage instructions Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Storage buffer Preservative: 0.09% Sodium Azide
Concentration information loading...
Purity Immunogen affinity purified
Purification notes This antibody is purified through a protein A column, followed by peptide affinity purification.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Predicted molecular weight: 46 kDa.
Function ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
Sequence similarities Belongs to the DEAD box helicase family. eIF4A subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Information by UniProt
ATP dependent RNA helicase eIF4A 2 antibody
ATP-dependent RNA helicase eIF4A-2 antibody
Anti-eIF4A2 antibody images
Western blot - eIF4A2 antibody (ab95296)
Anti-eIF4A2 antibody (ab95296) at 1/100 dilution (TTBS ((Tween-Tris Buffered Saline: 0.1% Tween-20 in 100 mM Tris-Cl [pH 7.5], 0.9% NaCl) )/1% NFDM (nonfat dried milk)) + K562 cell lysate at 35 µg with 10% NFDM freshly made in TTBS Secondary Enzyme linked secondary antibody (typically horseradish peroxidase or alkaline phosphatase) in TTBS/1% NFDM for 30 min. at room temperature Predicted band size : 46 kDa
References for Anti-eIF4A2 antibody (ab95296)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"