Overview

  • Product nameAnti-eIF4B antibody
    See all eIF4B primary antibodies
  • Description
    Rabbit polyclonal to eIF4B
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cat
  • Immunogen

    Synthetic peptide: PTSGGGKVAP AQPSEEGPGR KDENKVDGMN APKGQTGNSS RGPGDGGNRD, corresponding to a region within C terminal amino acids 505-554 of Human eIF4B

  • Positive control
    • Jurkat cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 69 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • FunctionRequired for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
  • Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications
    Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2310046H11Rik antibody
    • AL024095 antibody
    • C85189 antibody
    • EIF 4B antibody
    • eIF-4B antibody
    • EIF4B antibody
    • Eukaryotic initiation factor 4B antibody
    • Eukaryotic translation initiation factor 4B antibody
    • IF4B_HUMAN antibody
    • PRO1843 antibody
    see all

Anti-eIF4B antibody images

  • Anti-eIF4B antibody (ab66780) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 69 kDa
    Observed band size : 63,65 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue labelling eIF4B with ab66780 at 4-8µg/ml. Arrows indicate positively labelled myocardial cells. Magnification: 400X.

References for Anti-eIF4B antibody (ab66780)

ab66780 has not yet been referenced specifically in any publications.

Product Wall

Thank you very much for contacting us regarding our eIF4B antibodies. We sell these three particular antibodies because they have been derived from different areas of human eIF4B. Ab78916 is derived from human eIF4B around the phosphorylation site ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"