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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.09% Sodium Azide
Concentration information loading...
Purity Immunogen affinity purified
Purification notes The antibody was purified through a protein A column, followed by peptide affinity purification.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/50.
1/50 - 1/100. Detects a band of approximately 79 kDa.
Function Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Sequence similarities Contains 1 RRM (RNA recognition motif) domain.
Post-translational modifications Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex.
Information by UniProt
Anti-eIF4B antibody images
Western blot - eIF4B antibody (ab71057)
All lanes : Anti-eIF4B antibody (ab71057) at 1/50 dilution Lane 1 : 293 cell lysate, non transfected Lane 2 : 293 cell lysate, transiently transfected with the eIF4B gene Lysates/proteins at 2 µg per lane. Observed band size : 79 kDa ( why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - eIF4B antibody (ab71057)
ab71057, at a dilution of 1/50, staining eIF4B in formalin fixed, paraffin embedded human breast carcinoma tissue (peroxidase conjugated secondary antibody, DAB staining).
References for Anti-eIF4B antibody (ab71057)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"