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Product name Anti-eIF4B (phospho S504) antibody [EP2261Y] See all eIF4B primary antibodies
Rabbit monoclonal [EP2261Y] to eIF4B (phospho S504)
Specificity ab75823 detects eIF4B phosphorylated at serine 504.
Tested applications Suitable for:
WB, ICC more details Unsuitable for:
Flow Cyt,IHC-P or IP
Mouse, Rat, Human
A phospho specific peptide corresponding to residues surrounding S504 of human eIF4B
293T cell lysates, untreated or treated with AP.
Produced using Abcam’s RabMAb
® technology. RabMAb ® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Storage instructions Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity Tissue culture supernatant
Clone number EP2261Y
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/25000 - 1/50000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
1/100 - 1/250.
Application notes Is unsuitable for Flow Cyt,IHC-P or IP.
Function Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Sequence similarities Contains 1 RRM (RNA recognition motif) domain.
Post-translational modifications Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex.
Information by UniProt
Anti-eIF4B (phospho S504) antibody [EP2261Y] images
Western blot - eIF4B (phospho S504) antibody [EP2261Y] (ab75823)
All lanes : Anti-eIF4B (phospho S504) antibody [EP2261Y] (ab75823) at 1/50000 dilution Lane 1 : 293T cell lysate, untreated Lane 2 : 293T cell lysate, treated with AP Lysates/proteins at 10 µg per lane. Secondary goat anti-rabbit HRP at 1/2000 dilution Predicted band size : 69 kDa Observed band size : 69 kDa
References for Anti-eIF4B (phospho S504) antibody [EP2261Y] (ab75823)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"