Overview

  • Product name
    Anti-eIF4B (phospho S504) antibody [EP2261Y]
    See all eIF4B primary antibodies
  • Description
    Rabbit monoclonal [EP2261Y] to eIF4B (phospho S504)
  • Specificity
    ab75823 detects eIF4B phosphorylated at serine 504.
  • Tested applications
    Suitable for: WB, ICCmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding S504 of human eIF4B

  • Positive control
    • 293T cell lysates, untreated or treated with AP.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EP2261Y
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/25000 - 1/50000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
    • Sequence similarities
      Contains 1 RRM (RNA recognition motif) domain.
    • Post-translational
      modifications
      Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2310046H11Rik antibody
      • AL024095 antibody
      • C85189 antibody
      • EIF 4B antibody
      • eIF-4B antibody
      • EIF4B antibody
      • Eukaryotic initiation factor 4B antibody
      • Eukaryotic translation initiation factor 4B antibody
      • IF4B_HUMAN antibody
      • PRO1843 antibody
      see all

    Images

    • All lanes : Anti-eIF4B (phospho S504) antibody [EP2261Y] (ab75823) at 1/50000 dilution

      Lane 1 : 293T cell lysate, untreated
      Lane 2 : 293T cell lysate, treated with AP

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 69 kDa
      Observed band size : 69 kDa

    References

    This product has been referenced in:
    • Bettegazzi B  et al. eIF4B phosphorylation at Ser504 links synaptic activity with protein translation in physiology and pathology. Sci Rep 7:10563 (2017). Read more (PubMed: 28874824) »

    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab75823.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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