Anti-eIF4B (phospho S504) antibody [EP2261Y] (ab75823)

Overview

  • Product nameAnti-eIF4B (phospho S504) antibody [EP2261Y]
    See all eIF4B primary antibodies
  • Description
    Rabbit monoclonal [EP2261Y] to eIF4B (phospho S504)
  • Specificityab75823 detects eIF4B phosphorylated at serine 504.
  • Tested applicationsSuitable for: WB, ICCmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding S504 of human eIF4B

  • Positive control
    • 293T cell lysates, untreated or treated with AP.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEP2261Y
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/25000 - 1/50000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
ICC 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • FunctionRequired for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
    • Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
    • Post-translational
      modifications
      Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2310046H11Rik antibody
      • AL024095 antibody
      • C85189 antibody
      • EIF 4B antibody
      • eIF-4B antibody
      • EIF4B antibody
      • Eukaryotic initiation factor 4B antibody
      • Eukaryotic translation initiation factor 4B antibody
      • IF4B_HUMAN antibody
      • PRO1843 antibody
      see all

    Anti-eIF4B (phospho S504) antibody [EP2261Y] images

    • All lanes : Anti-eIF4B (phospho S504) antibody [EP2261Y] (ab75823) at 1/50000 dilution

      Lane 1 : 293T cell lysate, untreated
      Lane 2 : 293T cell lysate, treated with AP

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 69 kDa
      Observed band size : 69 kDa

    References for Anti-eIF4B (phospho S504) antibody [EP2261Y] (ab75823)

    ab75823 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"