Overview

  • Product name
    Anti-eIF4E antibody [5D11]
    See all eIF4E primary antibodies
  • Description
    Mouse monoclonal [5D11] to eIF4E
  • Tested applications
    Suitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Rat
  • Immunogen

    Recombinant full length protein corresponding to Human eIF4E. Produced in HEK293T cells.
    Database link: P06730

  • Positive control
    • Hela, MCF7, K562, Jurkat, U2OS and HepG2 lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab171091 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/500.
WB 1/1000. Predicted molecular weight: 25 kDa.
IP Use at 6 µg/mg of lysate.

Target

  • Function
    Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
  • Sequence similarities
    Belongs to the eukaryotic initiation factor 4E family.
  • Post-translational
    modifications
    Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • AUTS19 antibody
    • CBP antibody
    • eIF 4E antibody
    • eIF 4F 25 kDa subunit antibody
    • EIF 4F antibody
    • eIF-4E antibody
    • eIF-4F 25 kDa subunit antibody
    • eIF4E antibody
    • EIF4E1 antibody
    • EIF4EL1 antibody
    • EIF4F antibody
    • Eukaryotic translation initiation factor 4 E antibody
    • Eukaryotic translation initiation factor 4E antibody
    • Eukaryotic translation initiation factor 4E like 1 antibody
    • IF4E_HUMAN antibody
    • Messanger RNA Cap Binding Protein eIF 4E antibody
    • MGC111573 antibody
    • mRNA cap binding protein antibody
    • mRNA cap-binding protein antibody
    see all

Images

  • Immunoprecipitation of eIF4E from HeLa whole cell lysate using ab171091 at 3µg per 500µg lysate.

     

    Lane 1

    Primary: ab117091, 1/1000 dilution
    Sample: Hela whole cell lysate

  • ab171091 staining eIF4E (green) in the cytoplasm of MCF-7 cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated goat-anti-mouse IgG (H+L) secondary antibody was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.

  • ab171091 staining eIF4E (green) in the cytoplasm of Hela cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated goat-anti-mouse IgG (H+L) secondary antibody was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.

  • All lanes : Anti-eIF4E antibody [5D11] (ab171091) at 1/1000 dilution

    Lane 1 : MCF7 whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : K562 whole cell lysate
    Lane 4 : Jurkat whole cell lysate
    Lane 5 : U2OS whole cell lysate
    Lane 6 : HepG2 whole cell lysate
    Lane 7 : C2C12 whole cell lysate
    Lane 8 : NIH3T3 whole cell lysate
    Lane 9 : NRK whole cell lysate

    Lysates/proteins at 80 µg per lane.

    Secondary
    Goat anti-mouse-HRP at 1/20000 dilution
    Developed using the ECL technique

    Predicted band size : 25 kDa

References

ab171091 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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