Overview

  • Product nameAnti-eIF4E antibody
    See all eIF4E primary antibodies
  • Description
    Rabbit polyclonal to eIF4E
  • Tested applicationsSuitable for: IP, WB, IHC-P, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab1126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/1000.
WB 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 25 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/1000.
ICC/IF Use at an assay dependent concentration. See Abreview.

Target

  • FunctionIts translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
  • Sequence similaritiesBelongs to the eukaryotic initiation factor 4E family.
  • Post-translational
    modifications
    Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • AUTS19 antibody
    • CBP antibody
    • eIF 4E antibody
    • eIF 4F 25 kDa subunit antibody
    • EIF 4F antibody
    • eIF-4E antibody
    • eIF-4F 25 kDa subunit antibody
    • eIF4E antibody
    • EIF4E1 antibody
    • EIF4EL1 antibody
    • EIF4F antibody
    • Eukaryotic translation initiation factor 4 E antibody
    • Eukaryotic translation initiation factor 4E antibody
    • Eukaryotic translation initiation factor 4E like 1 antibody
    • IF4E_HUMAN antibody
    • Messanger RNA Cap Binding Protein eIF 4E antibody
    • MGC111573 antibody
    • mRNA cap binding protein antibody
    • mRNA cap-binding protein antibody
    see all

Anti-eIF4E antibody images

  • Anti-eIF4E antibody (ab1126) + 30ug bovine kidney extract

    Predicted band size : 25 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
  • ab1126 at 1/100 staining human epithelial cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with BSA prior to incubation with the antibody for 1 hour. An Alexa-Fluor ® 647 conjugated goat anti-rabbit IgG was used as the secondary.

    See Abreview

  • IHC image of ab1126 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1126, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-eIF4E antibody (ab1126)

This product has been referenced in:
  • Zhang P  et al. p53, MDM2, eIF4E and EGFR expression in nasopharyngeal carcinoma and their correlation with clinicopathological characteristics and prognosis: A retrospective study. Oncol Lett 9:113-118 (2015). IHC ; Human . Read more (PubMed: 25435943) »
  • Mittal S  et al. The Ccr4a (CNOT6) and Ccr4b (CNOT6L) deadenylase subunits of the human Ccr4-Not complex contribute to the prevention of cell death and senescence. Mol Biol Cell 22:748-58 (2011). Read more (PubMed: 21233283) »

See all 4 Publications for this product

Product Wall

Thank you for your response.
This is to let you know that I have just contacted and asked our Account Department to raise a credit note for you - for the cost of two vials of ab1126. For your information, the internal reference note for this credi...

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Thank you for contacting us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. As it can be seen from the image in the datasheet, human breast carcinoma is a good option for a positive control. Howev...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLA)
Loading amount 20 µg
Specification HeLA
Gel Running Conditions Reduced Denaturing (15% Tris-tricine gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Sep 06 2011

Thank you for your enquiry regarding ab1126. I can confirm that the antibody has been tested in IHC-P at a dilution of 1/1000 (instead of 1ug/ml). The datasheet (application notes) and the legend of the IHC-P image has been amended accordingly. ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Epithelial)
Specification Epithelial
Fixative Paraformaldehyde
Permeabilization Yes - Triton-X 100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jun 05 2007

Thank you for your enquiry. I can confirm that both these eIF2a antibodies (ab1126, ab5369)will detect both phosphorylated and de-phosphorylated forms of the protein. I hope this information is helpful. Should you have any further questions, pl...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Blocking step 1% BSA + 1.5% normal goat serum as blocking agent for 30 minute(s) · Concentration: 1
Username

Dr. Karppiah Muthumani

Verified customer

Submitted Nov 17 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (HeLa)
Loading amount 50 µg
Specification HeLa
Treatment rVpr & LMB
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5
Username

Dr. Karppiah Muthumani

Verified customer

Submitted Nov 17 2006

Thank you very much for your e-mail. I would like to reassure you that if an antibody does not perform as specified on the datasheet, we will provide a full refund or offer you a replacement of equivalent value. I have contacted the supplier reg...

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Yes, our antibody is capable of precipitating eIF-4E from cell lysates. The antibody was made to the full length recombinant human protein.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"