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Our Abpromise guarantee covers the use of ab1126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 25 kDa).|
|IHC-P||1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent concentration. See Abreview.|
ab1126 at 1/100 staining human epithelial cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with BSA prior to incubation with the antibody for 1 hour. An Alexa-Fluor ® 647 conjugated goat anti-rabbit IgG was used as the secondary.