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Rabbit polyclonal to eIF4E
ab47482 detects endogenous levels of total elF4E protein.
Mouse, Rat, Human
Synthetic non-phosphopeptide derived from human elF4E around the phosphorylation site of serine 209 (S-G-S
Breast carcinoma tissue. NIH/3T3 cells extract.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
1/500 - 1/1000. Predicted molecular weight: 25 kDa.
Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Belongs to the eukaryotic initiation factor 4E family.
Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
Information by UniProt
eIF 4E antibody
Anti-eIF4E antibody images
Immunohistochemistry (Paraffin-embedded sections) - eIF4E antibody (ab47482)
Immunohistochemical analysis of elF4E expression on paraffin-embedded human breast carcinoma tissue using ab47482 at a 1/50 dilution. Right panel: the Ab was incubated with the immunising peptide.
Western blot - eIF4E antibody (ab47482)
All lanes : Anti-eIF4E antibody (ab47482) at 1/500 dilution Lane 1 : FBS treated NIH/3T3 cells extract Lane 2 : FBS treated NIH/3T3 cells extract with blocking peptide Predicted band size : 25 kDa Observed band size : 25 kDa
References for Anti-eIF4E antibody (ab47482)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"