For the best experience on the Abcam website please upgrade to a modern browser such as
Product name Anti-eIF4E antibody See all eIF4E primary antibodies
Rabbit polyclonal to eIF4E
Specificity ab47482 detects endogenous levels of total elF4E protein.
Tested applications Suitable for:
ELISA, IHC-P, WB more details
Mouse, Rat, Human
Synthetic non-phosphopeptide derived from human elF4E around the phosphorylation site of serine 209 (S-G-S
Breast carcinoma tissue. NIH/3T3 cells extract.
Storage instructions Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Purity Immunogen affinity purified
Purification notes The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
1/500 - 1/1000. Predicted molecular weight: 25 kDa.
Function Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Sequence similarities Belongs to the eukaryotic initiation factor 4E family.
Post-translational modifications Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
Information by UniProt
eIF 4E antibody
Anti-eIF4E antibody images
Immunohistochemistry (Paraffin-embedded sections) - eIF4E antibody (ab47482)
Immunohistochemical analysis of elF4E expression on paraffin-embedded human breast carcinoma tissue using ab47482 at a 1/50 dilution. Right panel: the Ab was incubated with the immunising peptide.
Western blot - eIF4E antibody (ab47482)
All lanes : Anti-eIF4E antibody (ab47482) at 1/500 dilution Lane 1 : FBS treated NIH/3T3 cells extract Lane 2 : FBS treated NIH/3T3 cells extract with blocking peptide Predicted band size : 25 kDa Observed band size : 25 kDa
References for Anti-eIF4E antibody (ab47482)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"