Anti-eIF4E antibody (ab72116)
Key features and details
- Rabbit polyclonal to eIF4E
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-eIF4E antibody
See all eIF4E primary antibodies -
Description
Rabbit polyclonal to eIF4E -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Elephant -
Immunogen
Synthetic peptide corresponding to a region between residue 1 and 50 of human eIF4E (NP_001959.1).
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Positive control
- HeLa whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab72116 was affinity purified using an epitope specific to eIF4E immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 2-5 µg/mg of lysate.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
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Notes |
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IP
Use at 2-5 µg/mg of lysate. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa. |
Target
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Function
Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap. -
Sequence similarities
Belongs to the eukaryotic initiation factor 4E family. -
Post-translational
modificationsPhosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex. - Information by UniProt
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Database links
- Entrez Gene: 281751 Cow
- Entrez Gene: 1977 Human
- Entrez Gene: 13684 Mouse
- Omim: 133440 Human
- SwissProt: Q9N0T5 Cow
- SwissProt: P06730 Human
- SwissProt: P63073 Mouse
- SwissProt: P29338 Rabbit
see all -
Alternative names
- AUTS19 antibody
- CBP antibody
- eIF 4E antibody
see all
Images
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All lanes : Anti-eIF4E antibody (ab72116) at 0.1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : HEK293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : TCMK-1 cell lysate
Lane 5 : NIH3T3 cell lysate
Predicted band size: 25 kDaDetection: Chemiluminescence with an exposure time of 10 seconds.
Previous -
Detection of eIF4E by Western Blot of Immunprecipitate.
anti-eIF4E at 1µg/ml staining eIF4E in HeLa whole cell lysate immunoprecipitated using ab72116 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab72116 has not yet been referenced specifically in any publications.