Overview

  • Product name
  • Description
    Rabbit polyclonal to eIF4E
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1 and 50 of human eIF4E (NP_001959.1).

  • Positive control
    • HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab72116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
    • Sequence similarities
      Belongs to the eukaryotic initiation factor 4E family.
    • Post-translational
      modifications
      Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
    • Information by UniProt
    • Database links
    • Alternative names
      • AUTS19 antibody
      • CBP antibody
      • eIF 4E antibody
      • eIF 4F 25 kDa subunit antibody
      • EIF 4F antibody
      • eIF-4E antibody
      • eIF-4F 25 kDa subunit antibody
      • eIF4E antibody
      • EIF4E1 antibody
      • EIF4EL1 antibody
      • EIF4F antibody
      • Eukaryotic translation initiation factor 4 E antibody
      • Eukaryotic translation initiation factor 4E antibody
      • Eukaryotic translation initiation factor 4E like 1 antibody
      • IF4E_HUMAN antibody
      • Messanger RNA Cap Binding Protein eIF 4E antibody
      • MGC111573 antibody
      • mRNA cap binding protein antibody
      • mRNA cap-binding protein antibody
      see all

    Images

    • Detection of eIF4E by Western Blot of Immunprecipitate.
      anti-eIF4E at 1µg/ml staining eIF4E in HeLa whole cell lysate immunoprecipitated using ab72116 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 3 seconds.

    References

    ab72116 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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