The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
Application notesIs unsuitable for WB.
FunctionIts translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Sequence similaritiesBelongs to the eukaryotic initiation factor 4E family.
Post-translational modificationsPhosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
Eukaryotic translation initiation factor 4 E antibody
Eukaryotic translation initiation factor 4E antibody
Eukaryotic translation initiation factor 4E like 1 antibody
Messanger RNA Cap Binding Protein eIF 4E antibody
mRNA cap binding protein antibody
mRNA cap-binding protein antibody
Anti-eIF4E antibody images
Immunoprecipitation - eIF4E antibody (ab72116)
Detection of eIF4E by Western Blot of Immunprecipitate.
anti-eIF4E at 1µg/ml staining eIF4E in HeLa whole cell lysate immunoprecipitated using ab72116 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 seconds.
References for Anti-eIF4E antibody (ab72116)
has not yet been referenced specifically in any publications.
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