Recombinant
RabMAb

Anti-eIF4E antibody [Y449] (ab33768)

Overview

  • Product name
    Anti-eIF4E antibody [Y449]
    See all eIF4E primary antibodies
  • Description
    Rabbit monoclonal [Y449] to eIF4E
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, IPmore details
    Unsuitable for: ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in the C terminus of Human elf 4E.

  • Positive control
    • WB: 293 cell lysate. IHC-P: Human breast carcinoma.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab33768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Detects a band of approximately 30 kDa (predicted molecular weight: 25 kDa).
IHC-P Use at an assay dependent concentration.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/40.
  • Application notes
    Is unsuitable for ICC.
  • Target

    • Function
      Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
    • Sequence similarities
      Belongs to the eukaryotic initiation factor 4E family.
    • Post-translational
      modifications
      Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
    • Information by UniProt
    • Database links
    • Alternative names
      • AUTS19 antibody
      • CBP antibody
      • eIF 4E antibody
      • eIF 4F 25 kDa subunit antibody
      • EIF 4F antibody
      • eIF-4E antibody
      • eIF-4F 25 kDa subunit antibody
      • eIF4E antibody
      • EIF4E1 antibody
      • EIF4EL1 antibody
      • EIF4F antibody
      • Eukaryotic translation initiation factor 4 E antibody
      • Eukaryotic translation initiation factor 4E antibody
      • Eukaryotic translation initiation factor 4E like 1 antibody
      • IF4E_HUMAN antibody
      • Messanger RNA Cap Binding Protein eIF 4E antibody
      • MGC111573 antibody
      • mRNA cap binding protein antibody
      • mRNA cap-binding protein antibody
      see all

    Images

    • Ab33768, at 1/250 dilution, staining eIF4E in paraffin embedded human breast carcinoma tissue sections by Immunohistochemistry.
    • Overlay histogram showing HEK293 cells stained with ab33768 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab33768, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Anti-eIF4E antibody [Y449] (ab33768) at 1/20000 dilution + 293 cell lysate

      Predicted band size : 25 kDa
      Observed band size : 30 kDa (why is the actual band size different from the predicted?)

    References

    ab33768 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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