Anti-eIF4E (phospho S209) antibody (ab131513)
Key features and details
- Rabbit polyclonal to eIF4E (phospho S209)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-eIF4E (phospho S209) antibody
See all eIF4E primary antibodies -
Description
Rabbit polyclonal to eIF4E (phospho S209) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human eIF4E (internal sequence) (phospho S209) conjugated to keyhole limpet haemocyanin. NP_001124150.1
Database link: P06730 -
Positive control
- Methanol fixed MCF cells; HT29 cell extracts treated with Anisomycin; Human breast carcinoma tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab131513 was purified using epitope specific phosphopeptide. Non phospho specific antibodies were removed by chromatography using non phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131513 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 25 kDa.
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IHC-P |
1/50 - 1/100.
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ICC/IF |
1/100 - 1/200.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 25 kDa. |
IHC-P
1/50 - 1/100. |
ICC/IF
1/100 - 1/200. |
Target
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Function
Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap. -
Sequence similarities
Belongs to the eukaryotic initiation factor 4E family. -
Post-translational
modificationsPhosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex. - Information by UniProt
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Database links
- Entrez Gene: 1977 Human
- Omim: 133440 Human
- SwissProt: P06730 Human
- Unigene: 249718 Human
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Alternative names
- AUTS19 antibody
- CBP antibody
- eIF 4E antibody
see all
Images
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All lanes : Anti-eIF4E (phospho S209) antibody (ab131513) at 1/500 dilution
Lane 1 : HT29 cell extracts, untreated
Lane 2 : HT29 cell extracts Anisomycin treated
Predicted band size: 25 kDa
For western blotting, membrane should be incubated with diluted antibody in 5% nonfat milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking overnight. -
Immunofluorescence analysis of eIF4E (phospho S209) in methanol fixed MCF cells stained with ab131513 at a dilution of 1/100.
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Immunohistochemical analysis of eIF4E (phospho S209) in paraffin embedded Human breast carcinoma tissue stained with ab131513 at a 1/50 dilution (left image), or preincubated with immunizing peptide (right image).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab131513 has not yet been referenced specifically in any publications.