Anti-eIF4E (phospho S209) antibody (ab47605)

Overview

  • Product nameAnti-eIF4E (phospho S209) antibody
    See all eIF4E primary antibodies
  • Description
    Rabbit polyclonal to eIF4E (phospho S209)
  • SpecificityThis antibody is specific for elF4E, only when phosphorylated at serine 209.
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    A synthesized phosphopeptide derived from human elF4E around the phosphorylation site of serine 209 (S-G-SP-T-T)

  • Positive control
    • WB: Extracts from NIH/3T3 cells IHC: Human breast carcinoma tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab47605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • FunctionIts translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
  • Sequence similaritiesBelongs to the eukaryotic initiation factor 4E family.
  • Post-translational
    modifications
    Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • AUTS19 antibody
    • CBP antibody
    • eIF 4E antibody
    • eIF 4F 25 kDa subunit antibody
    • EIF 4F antibody
    • eIF-4E antibody
    • eIF-4F 25 kDa subunit antibody
    • eIF4E antibody
    • EIF4E1 antibody
    • EIF4EL1 antibody
    • EIF4F antibody
    • Eukaryotic translation initiation factor 4 E antibody
    • Eukaryotic translation initiation factor 4E antibody
    • Eukaryotic translation initiation factor 4E like 1 antibody
    • IF4E_HUMAN antibody
    • Messanger RNA Cap Binding Protein eIF 4E antibody
    • MGC111573 antibody
    • mRNA cap binding protein antibody
    • mRNA cap-binding protein antibody
    see all

Anti-eIF4E (phospho S209) antibody images

  • All lanes : Anti-eIF4E (phospho S209) antibody (ab47605) at 1/500 dilution

    Lane 1 : Extracts from NIH/3T3 cells, treated with FBS. No peptide.
    Lane 2 : Extracts from NIH/3T3 cells, not treated with FBS. No peptide.

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 25 kDa
    Observed band size : 25 kDa
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab47605 at a 1/50 dilution.

References for Anti-eIF4E (phospho S209) antibody (ab47605)

ab47605 has not yet been referenced specifically in any publications.

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