• Product nameAnti-eIF4EBP1 antibody
    See all eIF4EBP1 primary antibodies
  • Description
    Rabbit polyclonal to eIF4EBP1
  • SpecificityThis antibody may cross-react with elF4EBP2 and elF4EBP3.
  • Tested applicationsSuitable for: ELISA, IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A synthesized non-phosphopeptide derived from human elf4EBP1 around the phosphorylation site of threonine 45 (S-T-TP-P-G).

  • Positive control
    • MDA-MB-435 cell extract and human breast carcinoma tissue


Associated products


Our Abpromise guarantee covers the use of ab47467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 13 kDa).
ICC/IF Use a concentration of 1 - 5 µg/ml.


  • FunctionRegulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
  • Sequence similaritiesBelongs to the eIF4E-binding protein family.
  • Post-translational
    Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4E-BP1 antibody
    • 4EBP1 antibody
    • 4EBP1_HUMAN antibody
    • BP 1 antibody
    • eIF4E binding protein 1 antibody
    • eIF4E-binding protein 1 antibody
    • Eif4ebp1 antibody
    • Eukaryotic translation initiation factor 4E-binding protein 1 antibody
    • PHAS-I antibody
    • PHASI antibody
    • Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody
    see all

Anti-eIF4EBP1 antibody images

  • All lanes : Anti-eIF4EBP1 antibody (ab47467) at 1/500 dilution

    Lane 1 : MDA-MB-435 cells treated with EGF (200ng/ml, 5min)
    Lane 2 : MDA-MB-435 cells treated with EGF (200ng/ml, 5min) and the immunogenic peptide.

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 13 kDa
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab47467 at a 1/50 dilution and Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) secondary antibody.
  • ICC/IF image of ab47467 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47467, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-eIF4EBP1 antibody (ab47467)

ab47467 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab47467.
Please use the links above to contact us or submit feedback about this product.