The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
1/500 - 1/1000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).
FunctionRegulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Sequence similaritiesBelongs to the eIF4E-binding protein family.
Post-translational modificationsPhosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
Eukaryotic translation initiation factor 4E-binding protein 1 antibody
Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody
Anti-eIF4EBP1 antibody images
Western blot - eIF4EBP1 antibody (ab63529)
All lanes : Anti-eIF4EBP1 antibody (ab63529) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells (5ug), treated
with Insulin (0.01U/ml, 15mins) Lane 2 : Extracts from Jurkat cells (5ug), treated
with Insulin (0.01U/ml, 15mins), with immunizing non-phosphopeptide at 5 µg
Predicted band size : 13 kDa Observed band size : 13 kDa