Anti-eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) antibody [EPR2169Y] (ab68187)


  • Product nameAnti-eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) antibody [EPR2169Y]
  • Description
    Rabbit monoclonal [EPR2169Y] to eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45)
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding threonine 45

  • Positive control
    • WB: 293T cell lysate IHC-P: Human breast carcinoma tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab68187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/10000. Detects a band of approximately 14-19 kDa (predicted molecular weight: 13 kDa).
IP 1/10.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • RelevanceeIF4EBP1 is a member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. eIF4EBP2 regulates eIF4E activity by preventing its assembly into the EIF4F complex. It mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase pathway. Nonphosphorylated EIF4EBP2 interacts with EIF4E. eIF4EBP2 is phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF, and belongs to the eIF4E binding protein family. eIF4EBP3 is a member of the EIF4EBP family which derives it name from proteins that bind to eukaryotic initiation factor 4E and that prevent its assembly into EIF4F. Cotranscription of this gene and the neighboring upstream gene (MASK) generates a transcript (MASK-BP3) which encodes a fusion protein comprised of the MASK protein sequence for the majority of the protein and a different C terminus due to an alternate reading frame for the EIF4EBP3 segments.
    • Database links
    • Alternative names
      • 4EBP1 antibody
      • 4EBP2 antibody
      • 4EBP3 antibody
      • eIF4E binding protein 1 antibody
      • eIF4E binding protein 2 antibody
      • eIF4E binding protein 3 antibody
      • Eukaryotic translation initiation factor 4E binding protein 1 antibody
      • Eukaryotic translation initiation factor 4E binding protein 2 antibody
      • Eukaryotic translation initiation factor 4E binding protein 3 antibody
      • PHAS1 antibody
      see all

    Anti-eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) antibody [EPR2169Y] images

    • All lanes : Anti-eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) antibody [EPR2169Y] (ab68187)

      Lane 1 : 293T cell lysate, untreated.
      Lane 2 : 293T cell lysate, treated with FBS.

      Lysates/proteins at 10 µg per lane.

      HRP conjugated Goat anti-rabbit at 1/2000 dilution

      Predicted band size : 13 kDa
      Observed band size : 14-19 kDa (why is the actual band size different from the predicted?)
    • Human breast carcinoma tissue stained with ab68187 at 1/50 dilution.

    References for Anti-eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) antibody [EPR2169Y] (ab68187)

    This product has been referenced in:
    • Wang S  et al. The role of autophagy in the neurotoxicity of cationic PAMAM dendrimers. Biomaterials 35:7588-97 (2014). Read more (PubMed: 24906346) »
    • Fan J  et al. Autophagy Plays a Critical Role in ChLym-1-Induced Cytotoxicity of Non-Hodgkin's Lymphoma Cells. PLoS One 8:e72478 (2013). WB ; Human . Read more (PubMed: 24015249) »

    See all 2 Publications for this product

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