Anti-eIF4EBP1 (phospho S64) antibody (ab53686)

Overview

  • Product nameAnti-eIF4EBP1 (phospho S64) antibody
    See all eIF4EBP1 primary antibodies
  • Description
    Rabbit polyclonal to eIF4EBP1 (phospho S64)
  • Specificityab53686 detects endogenous levels of eIF4EBP1 only when phosphorylated at serine 64.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from human eIF4EBP1 around the phosphorylation site of serine 64 (R-N-SP-P-V).

  • Positive control
    • Human colon carcinoma tissue and Jurkat cell extracts treated with Insulin (0.01U/ml, 15mins).

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab53686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).
ELISA 1/1000.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionRegulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
  • Sequence similaritiesBelongs to the eIF4E-binding protein family.
  • Post-translational
    modifications
    Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4E-BP1 antibody
    • 4EBP1 antibody
    • 4EBP1_HUMAN antibody
    • BP 1 antibody
    • eIF4E binding protein 1 antibody
    • eIF4E-binding protein 1 antibody
    • Eif4ebp1 antibody
    • Eukaryotic translation initiation factor 4E-binding protein 1 antibody
    • PHAS-I antibody
    • PHASI antibody
    • Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody
    see all

Anti-eIF4EBP1 (phospho S64) antibody images

  • All lanes : Anti-eIF4EBP1 (phospho S64) antibody (ab53686) at 1/500 dilution

    Lane 1 : Jurkat cell extract treated with Insulin (0.01U/ml, 15mins)
    Lane 2 : Jurkat cell extract treated with Insulin (0.01U/ml, 15mins) with immunising peptide


    Predicted band size : 13 kDa
    Observed band size : 13 kDa
  • ab53686 at 1/50 dilution staining eIF4EBP1 in human colon carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

References for Anti-eIF4EBP1 (phospho S64) antibody (ab53686)

ab53686 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 27°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium citrate buffer
Sample Mouse Tissue sections (Brain tissue)
Specification Brain tissue
Permeabilization No
Fixative Acetone
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Submitted Mar 14 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 27°C
Sample Mouse Cell (Brain tissue)
Specification Brain tissue
Permeabilization No
Fixative Acetone
Username

Abcam user community

Verified customer

Submitted Mar 13 2014

Thank you for contacting us. Unfortunately we are unable to determine the percentage sequence homology between human and Litopenaeus vannamei eIF4BP1 sequence. So we will be unable to provide the discount code. We can however reconsider your reques...

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Thank you for contacting us. Could you specify the species you are interested in? Looking forward to hearing from you soon.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"