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Rabbit monoclonal [EPR729(2)Y] to eIF4EBP1 (phospho T37)
Detects eIF4EBP1 only when phosphorylated at threonine 37.
WB more details Unsuitable for:
Flow Cyt,ICC/IF,IHC-P or IP
A phospho-peptide corresponding to residues surrounding threonine 37 of eIF4EBP1.
This product is a recombinant rabbit monoclonal antibody.
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/2000. Predicted molecular weight: 13 kDa.
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Belongs to the eIF4E-binding protein family.
Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
Western blot - eIF4EBP1 (phospho T37) antibody [EPR729(2)Y] (ab75767)
All lanes :
Anti-eIF4EBP1 (phospho T37) antibody [EPR729(2)Y] (ab75767) at 1/2000 dilution
Lane 1 :
293 cell lysate, untreated
Lane 2 :
293 cell lysate, treated with insulin
Lysates/proteins at 10 µg per lane.
HRP-conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size :
Observed band size :
17 kDa (
why is the actual band size different from the predicted?
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"