Anti-eIF4EBP1 (phospho T45) antibody (ab47367)

Overview

  • Product nameAnti-eIF4EBP1 (phospho T45) antibody
    See all eIF4EBP1 primary antibodies
  • Description
    Rabbit polyclonal to eIF4EBP1 (phospho T45)
  • Specificityab47367 detects endogenous levels of 4EBP1 only when phosphorylated at threonine 45. This antibody may cross-react with 4EBP2 and 4EBP3 when phosphorylated at equivalent sites.
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    The antiserum was produced against synthesized phosphopeptide derived from human 4EBP1 around the phosphorylation site of threonine 45 (S-T-TP-P-G).

  • Positive control
    • MDA435 cells, breast carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab47367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 13 kDa.
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.

Target

  • FunctionRegulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
  • Sequence similaritiesBelongs to the eIF4E-binding protein family.
  • Post-translational
    modifications
    Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4E-BP1 antibody
    • 4EBP1 antibody
    • 4EBP1_HUMAN antibody
    • BP 1 antibody
    • eIF4E binding protein 1 antibody
    • eIF4E-binding protein 1 antibody
    • Eif4ebp1 antibody
    • Eukaryotic translation initiation factor 4E-binding protein 1 antibody
    • PHAS-I antibody
    • PHASI antibody
    • Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody
    see all

Anti-eIF4EBP1 (phospho T45) antibody images

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab47367. Left hand image without immunising peptide; right hand image with immunising peptide.


  • Predicted band size : 13 kDa

References for Anti-eIF4EBP1 (phospho T45) antibody (ab47367)

ab47367 has not yet been referenced specifically in any publications.

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