Anti-eIF4EBP1 (phospho T46) antibody (ab27792)

Overview

  • Product name
    Anti-eIF4EBP1 (phospho T46) antibody
    See all eIF4EBP1 primary antibodies
  • Description
    Rabbit polyclonal to eIF4EBP1 (phospho T46)
  • Specificity
    Ab27792 recognises eIF4EBP1 that is phosphorylated at threonine 46.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic phosphopeptide derived from the region of human eIF4EBP1 that contains threonine 46. The sequence is conserved in human, mouse and rat.

  • Positive control
    • Human embryonic kidney (HEK293) cells stimulated with EGF or 15% serum, or NIH3T3 cells stimulated with PDGF.

Properties

Applications

Our Abpromise guarantee covers the use of ab27792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 13 kDa.

Target

  • Function
    Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
  • Sequence similarities
    Belongs to the eIF4E-binding protein family.
  • Post-translational
    modifications
    Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4E-BP1 antibody
    • 4EBP1 antibody
    • 4EBP1_HUMAN antibody
    • BP 1 antibody
    • eIF4E binding protein 1 antibody
    • eIF4E-binding protein 1 antibody
    • Eif4ebp1 antibody
    • Eukaryotic translation initiation factor 4E-binding protein 1 antibody
    • PHAS-I antibody
    • PHASI antibody
    • Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody
    see all

Images

  • All lanes : Anti-eIF4EBP1 (phospho T46) antibody (ab27792) at 1/1000 dilution (diluted in 3% BSA TBST buffer, incubated for two hours at room temperature.)

    Lane 1 : PDGF treated NIH3T3 cell lysate
    Lane 2 : PI3K inhibitor LY2904002 treated NIH3T3 cell lysate
    Lane 3 : PI3K inhibitor LY2904002 and PDGF treated NIH3T3 cell lysate

    Secondary
    goat F(ab)2 anti rabbit IgG HRP conjugate

    Predicted band size : 13 kDa
    Observed band size : 18 kDa (why is the actual band size different from the predicted?)

References

ab27792 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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