Anti-eIF4EBP1 (phospho T70) antibody (ab111421)

Overview

  • Product nameAnti-eIF4EBP1 (phospho T70) antibody
    See all eIF4EBP1 primary antibodies
  • Description
    Rabbit polyclonal to eIF4EBP1 (phospho T70)
  • SpecificityDetects endogenous levels of eIF4EBP1 only when phosphorylated at threonine70.
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from human eIF4EBP1 (Q13541) around the phosphorylation site of Thr70 (T-K-TP-P-P).

  • Positive control
    • Human colon carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab111421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionRegulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
  • Sequence similaritiesBelongs to the eIF4E-binding protein family.
  • Post-translational
    modifications
    Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4E-BP1 antibody
    • 4EBP1 antibody
    • 4EBP1_HUMAN antibody
    • BP 1 antibody
    • eIF4E binding protein 1 antibody
    • eIF4E-binding protein 1 antibody
    • Eif4ebp1 antibody
    • Eukaryotic translation initiation factor 4E-binding protein 1 antibody
    • PHAS-I antibody
    • PHASI antibody
    • Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody
    see all

Anti-eIF4EBP1 (phospho T70) antibody images

  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using eIF4EBP1 (Phospho-Thr70) Antibody (ab111421). The picture on the right is treated with the synthesized phospho peptide.

References for Anti-eIF4EBP1 (phospho T70) antibody (ab111421)

ab111421 has not yet been referenced specifically in any publications.

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