Anti-eIF4EBP1 (phospho T70) antibody [EPR654(2)A] (ab75831)

Overview

  • Product nameAnti-eIF4EBP1 (phospho T70) antibody [EPR654(2)A]
    See all eIF4EBP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR654(2)A] to eIF4EBP1 (phospho T70)
  • Specificityab75831 detects eIF4EBP1 phosphorylated at threonine 70.
  • Tested applicationsSuitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding threonine 70 of human eIF4EBP1.

  • Positive control
    • 293T cell lysates treated with FBS.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab75831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 13 kDa).
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionRegulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
    • Sequence similaritiesBelongs to the eIF4E-binding protein family.
    • Post-translational
      modifications
      Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Information by UniProt
    • Database links
    • Alternative names
      • 4E-BP1 antibody
      • 4EBP1 antibody
      • 4EBP1_HUMAN antibody
      • BP 1 antibody
      • eIF4E binding protein 1 antibody
      • eIF4E-binding protein 1 antibody
      • Eif4ebp1 antibody
      • Eukaryotic translation initiation factor 4E-binding protein 1 antibody
      • PHAS-I antibody
      • PHASI antibody
      • Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody
      see all

    Anti-eIF4EBP1 (phospho T70) antibody [EPR654(2)A] images

    • All lanes : Anti-eIF4EBP1 (phospho T70) antibody [EPR654(2)A] (ab75831) at 1/500 dilution

      Lane 1 : 293T cell lysates un-treated
      Lane 2 : 293T cell lysates treated with FBS

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 13 kDa
      Observed band size : 17 kDa (why is the actual band size different from the predicted?)

    References for Anti-eIF4EBP1 (phospho T70) antibody [EPR654(2)A] (ab75831)

    ab75831 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"