Overview

  • Product nameAnti-EIF4EBP3 antibody
    See all EIF4EBP3 primary antibodies
  • Description
    Mouse monoclonal to EIF4EBP3
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment: MSTSTSCPIP GGRDQLPDCY STTPGGTLYA TTPGGTRIIY DRKFLLECKN SPIARTPPCC LPQIPGVTTP PTAPLSKLEE LKEQETEEEI PDDAQFEMDI , corresponding to amino acids 1-101 of Human EIF4EBP3 (NP_003723) with tag.

  • Positive control
    • EIF4EBP3 transfected 293T cell lysate. Recombinant tagged EIF4EBP3 protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged EIF4EBP3 is approximately 100ng/ml if used as a capture antibody.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 11 kDa.

Target

Anti-EIF4EBP3 antibody images

  • Anti-EIF4EBP3 antibody (ab76476) at 1 µg/ml + Recombinant tagged EIF4EBP3 protein at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 11 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-EIF4EBP3 antibody (ab76476) at 1 µg/ml

    Lane 1 : EIF4EBP3 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 11 kDa
    Observed band size : 16 kDa (why is the actual band size different from the predicted?)

References for Anti-EIF4EBP3 antibody (ab76476)

ab76476 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"