Overview

  • Product nameAnti-EIF4EL3 antibody
    See all EIF4EL3 primary antibodies
  • Description
    Mouse monoclonal to EIF4EL3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-246 of Human EIF4EL3

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab56637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 28 kDa.

Target

  • FunctionRecognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
  • Sequence similaritiesBelongs to the eukaryotic initiation factor 4E family.
  • Post-translational
    modifications
    Ubiquitinated by ARIH1, leading to its degradation by the proteasome.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4E LP antibody
    • 4EHP antibody
    • 4ELP antibody
    • EIF 4E type 2 antibody
    • eIF-4E type 2 antibody
    • EIF4 E2 antibody
    • EIF4E 2 antibody
    • eIF4E family, member 2 antibody
    • EIF4E HOMOLOGOUS PROTEIN antibody
    • EIF4E like cap binding protein antibody
    • EIF4E like protein 4E LP antibody
    • EIF4E like protein 4ELP antibody
    • eIF4E type 2 antibody
    • EIF4E type 3 antibody
    • eIF4E-like 3 antibody
    • eIF4E-like protein 4E-LP antibody
    • EIF4E2 antibody
    • Eukaryotic translation initiation factor 4E family member 2 antibody
    • Eukaryotic translation initiation factor 4E homologous protein antibody
    • Eukaryotic translation initiation factor 4E like 3 antibody
    • Eukaryotic translation initiation factor 4E member 2 antibody
    • Eukaryotic translation initiation factor 4E type 2 antibody
    • Eukaryotic translation initiation factor 4E-like 3 antibody
    • IF 4e antibody
    • IF4E antibody
    • IF4E2_HUMAN antibody
    • mRNA cap binding protein 4EHP antibody
    • mRNA cap binding protein type 3 antibody
    • mRNA cap-binding protein 4EHP antibody
    • mRNA cap-binding protein type 3 antibody
    see all

Anti-EIF4EL3 antibody images



  • Predicted band size : 28 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: EIF4EL3 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: HepG2 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab56637 observed at 28 kDa. Red - loading control, ab181602, observed at 37 kDa.
    ab56637 was shown to recognize EIF4EL3 when EIF4EL3 knockout samples were used, along with additional cross-reactive bands. Wild-type and EIF4EL3 knockout samples were subjected to SDS-PAGE. ab56637 and ab181602 (loading control to GAPDH) were diluted 5 µg/mL and 1/10 000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.



  • Predicted band size : 28 kDa
    EIF4EL3 antibody (ab56637) at 1ug/lane + HeLa cell lysate at 25ug/lane.

References for Anti-EIF4EL3 antibody (ab56637)

ab56637 has not yet been referenced specifically in any publications.

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